Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Potato Malt HiVeg Agar is used for cultivation and maintenance of smut fungi and other phytopathogenic fungi.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Potatoes, infusion from | 200.0 |
| Malt extract | 20.0 |
| HiVeg peptone | 1.0 |
| Sucrose | 60.0 |
| Agar | 20.0 |
Final pH (at 25°C) 7.4 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 10.5 grams in 100 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile petri plates.
Principle and Interpretation
Potato Malt HiVeg Agar is prepared by using HiVeg peptone in place of Peptic digest of animal tissue which makes the medium free of BSE/TSE risks. Potato Malt HiVeg Agar is the modification of Potato Malt Agar. Potato Malt HiVeg Agar is used for cultivating and maintaining smut fungi and other phytopathogenic fungi. In addition to smut fungi, this medium is also used for cultivation of other organisms causing plant disease and aciduric microorganisms which needs high carbohydrate content and neutral to slight alkaline pH for optimal growth.
Malt extract which contains dextrin, maltose, a little glucose, along with the potato infusion in the medium promotes luxuriant growth of fungi (1). HiVeg peptone provides the nitrogenous compounds and trace minerals to the organisms. Sucrose supports the growth of microorganisms such as yeasts.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comaparable with 2.0% Agar gel.
Colour and Clarity: Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 10.5% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV404 | M404 |
| HiVeg peptone | Peptic digest of animal tissue |
Recommended for
Cultivation and maintenance of smut fungi and other phytopathogenic fungi
Reconstitution
| Quantity on preparation (500g) | 4.76 L |
| pH (25°C) | 7.4 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 40-48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Aspergillus niger (16404) | luxuriant |
| Candida albicans (10231) | luxuriant |
| Saccharomyces cerevisiae (9763) | luxuriant |
References
- Vanderzant C. and Splittstoesserd (Eds) 1992. Compendium of Methods for the Microbiological Examination of foods, 3rd ed., APHA, Wahington D.C.
| Product Name | Potato Malt HiVeg™ Agar |
|---|---|
| SKU | MV404 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R. M., 2004, Handbook of Microbiological Media, Lawrence C. Parks (Ed.), 3rd Edition, CRC Press. 2.Vanderzant C. and Splittstoesser D. F., (Eds), 1992, Compendium of Methods for the Microbiological Examination of Foods,3rd Ed., APHA, Washington D.C. |
| Customized Product Available | No |
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