Fluid Thioglycollate Medium w/ HiVeg™ Extract

Intended Use

Fluid Thioglycollate HiVeg Media are used for sterility testing of biologicals and for cultivation of aerobic and anaerobic organisms.

Composition

Ingredients	MV009 Grams/Litre	MV380 Grams/Litre
HiVeg hydrolysate	15.00	15.00
Yeast extract	5.00	5.00
HiVeg extract	-	5.00
Dextrose	5.50	5.50
Sodium chloride	2.50	2.50
L-Cystine	0.50	0.50
Sodium thioglycollate	0.50	0.50
Resazurin sodium	0.001	0.001
Agar	0.75	0.75

Final pH (at 25°C) MV009: 7.1 ± 0.2, MV380: 7.2 ± 0.2

** Formula adjusted, standardized to suit performance parameters

Product Profile

Vegetable based (Code MV)

MV009/MV380

HiVeg hydrolysate
HiVeg extract

Animal based (Code M)

M009/M380

Casein enzymic hydrolysate
Beef extract

Recommended for : Sterility testing of biologicals and for cultivation of aerobic and anaerobic organisms.

Reconstitution :

(MV009): 29.75 g/l
(MV380): 34.75 g/l

Quantity on preparation (500g) :

(MV009): 16.80 L
(MV380): 14.38 L

Quantity on preparation (100g) : (MV009): 3.36 L

pH (25°C) :

(MV009): 7.1 ± 0.2
(MV380): 7.2 ± 0.2

Supplement : None

Sterilization : 121°C / 15 minutes.

Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.

Directions

Suspend 29.75 grams of MV009 or 34.75 grams of MV380 in 1000 ml distilled water. Heat to boiling the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in a cool dark place preferably below 25°C.

Note: If more than the upper one-third of the medium has acquired a pink colour, the medium may be restored once by heating in a water bath or in free flowing steam until the pink colour disappears.

Principle and Interpretation

Fluid Thioglycollate HiVeg Media are specially developed from HiVeg hydrolysate and HiVeg extract to avoid BSE/TSE risks associated with animal origin peptone. These media are the modifications of medium formulated by Brewer (by adding a reducing agent and small amount of agar) (1) for rapid cultivation of aerobes as well as anaerobes and recommended by AOAC (2) for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. However, it is intended for the examination of clear liquid or water-soluble materials. This medium (MV380) is also used in the detection of viable bacteria in vaccines.

Dextrose, HiVeg hydrolysate, HiVeg extract, yeast extract, L-Cystine provide the growth factors necessary for bacterial multiplication. Sodium thioglycollate act as a reducing agent and neutralizes the antibacterial effect of mercurial preservatives and other heavy metal compounds which exert a bacteriostatic effect on the materials under examination. L-Cystine is a reducing agent, since it contain sulfhydryl group, which inactivate heavy metal compounds and maintain low redox potential, thereby supporting anarobics. By creating an environment with a low Eh, the reducing agents prevent the accumulation of peroxides which can be toxic to some organisms. Any increase in the oxygen content is indicated by a colour change of redox indicator, resazurin to red (3,4,5). The small amount of agar helps in maintaining low redox potential for stabilizing the medium (6).

Quality Control

Appearance of Powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.

Colour and Clarity
Light straw coloured, clear to very slightly opalescent solution with upper 10% or less medium pink on standing.

Reaction
Reaction of 2.97% w/v aqueous solution of MV009 is pH 7.1 ± 0.2 at 25°C.
Reaction of 3.47% w/v aqueous solution of MV380 is pH 7.2 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 48-72 hours.

Organisms (ATCC)	Inoculum (CFU)	Growth	Recovery
*Bacillus subtilis (6633)	10-100	luxuriant	>70%
*Candida albicans (10231)	10-100	luxuriant	>70%
*Clostridium sporogenes (11437)	10-100	luxuriant	>70%
*Micrococcus luteus (9341)	10-100	luxuriant	>70%
Neisseria meningitidis (13090)	10-100	luxuriant	>70%
Streptococcus pyogenes (19615)	10-100	luxuriant	>70%
*Bacteroides vulgatus (8482)	10-100	fair-good	>50%

Key : * = These cultures may be incubated at 25-30°C for 2-7 days.

Product Name	Fluid Thioglycollate Medium w/ HiVeg™ Extract
SKU	MV380
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Brewer, 1940, J. Am. Med. Assoc., 115:598. 2.U. S. Pharmacopeia, 2002, USP 25/NF 20, Asian Edition, United States Pharmacopeial Convention, Inc., Rockville, MD. 3.Cunniff P. (Ed.), 1995, Official Methods of Analysis of the Association of Official Analytical Chemists, 16th ed., AOAC,Washington, D.C. 4.Marshall, Gunnison and Luxen, 1940, Proc. Soc. Exp. Biol. Med., 43:672. 5.Nungester, Hood and Warren, 1943, Proc. Soc. Exp. Biol. Med., 52:287. 6.Portwood, 1944, J. Bact., 48:255. 7.MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of ,,Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. 8.Federal Register, 1992, Fed. Regist., 21:113.26.
Customized Product Available	No

Fluid Thioglycollate Medium w/ HiVeg™ Extract