Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.0 |
| HiVeg extract | 5.0 |
| Sodium chloride | 5.0 |
| Final pH (at 25°C) | 6.8 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 20 grams in 1000 ml distilled water. Heat to dissolve the medium completely. Dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Disinfectant Test HiVeg Broth is prepared by using HiVeg peptone and Hiveg extract in place of Peptic digest of animal tissue and Beef extract respectively making the medium free of BSE/TSE risks. Disinfectant Test HiVeg Broth is the modification of Disinfectant Test Broth (1, 2) used for testing disinfectants and especially for determining phenol coefficients of disinfectants.
The medium contains ingredients like HiVeg extract and HiVeg peptone which provide necessary nutrients to the growth of test organisms like Salmonella serotype Typhi, Pseudomonas aeruginosa and Staphylococcus aureus. Presence of sodium chloride balances the osmotic equilibrium.
Product Profile
Vegetable based (Code MV)
- MV354
- HiVeg peptone
- HiVeg extract
Animal based (Code M)
- M354
- Peptic digest of animal tissue
- Beef extract
| Recommended for | : Testing disinfectants |
|---|---|
| Reconstitution | : 20.0 g/l |
| Quantity on preparation (500g) | : 25.0 L |
| pH (25°C) | : 6.8 ± 0.2 |
| Supplement | : None |
| Sterilization | : 121°C / 15 minutes. |
| Storage | : Dry Medium Below 30°C, Prepared Medium 2 - 8°C. |
Quality Control
Appearance of powder
Light yellow coloured, may have slight greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Medium amber coloured, clear to slightly opalescent solution.
Reaction
Reaction of 2.0% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 35-37° for 18 - 24 hours. (Recovery on MacConeky HiVeg Agar MV082)
| Organisms (ATCC) | Inoculum (CFU) | Growth* | Growth# | Recovery# | Colour of Colony |
|---|---|---|---|---|---|
| Pseudomonas aeruginosa (27853) | 102-103 | good-luxuriant | good-luxuriant | >70% | colourless |
| Salmonella serotype Typhi (6539) | 102-103 | good-luxuriant | good-luxuriant | >70% | colourless |
| Staphylococcus aureus (25923) | 102-103 | good-luxuriant | good-luxuriant | >70% | pale pink-red |
KEY :
- * = in MV354
- # = on MV082 Macconkey HiVeg Agar
References
- Williams (Ed.), 2005, Official methods of Analysis of AOAC, 18th ed. AOAC, Washington D.C.
- Journal of AOAC, 1964, 47:176.
- Methods of Analysis AOAC, 1980, 13th ed.
| Product Name | Disinfectant Test HiVeg™ Broth |
|---|---|
| SKU | MV354 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Association of Analytical Chemists (AOAC), 1960, 9th ed., Published by AOAC International. 2.Journal of AOAC, 1964, 47:176. 3.Methods of Analysis AOAC, 1980, 13th ed. 4.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 5.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
Write Your Own Review
Nutrient Broth, AOAC
As low as
₹4,679.00
Synthetic Broth, AOAC (Wright and Mundy Broth)
As low as
₹6,620.00
Upsell Products
Letheen Broth
As low as
₹1,398.00
Letheen Broth Modified
As low as
₹1,398.00