Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Acetobacter HiVeg Agar with HiVeg Extract No.2 is used as a maintenance medium for glucose positive Acetobacter species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg extract No. 2 | 2.0 |
| HiVeg hydrolysate | 5.0 |
| Glucose | 20.0 |
| Calcium carbonate | 10.0 |
| Agar | 20.0 |
Final pH (at 25°C) 7.4 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 57 grams in 1000 ml distilled water. Heat just to boiling. Dispense in test tubes taking care to distribute calcium carbonate evenly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Shake the tubes, cool quickly and place them in slanting position so as to keep the calcium carbonate in suspension.
Note: Due to presence of calcium carbonate, the prepared medium forms opalescent solution with white precipitate
Principle and Interpretation
Acetobacter HiVeg Agar with HiVeg Extract No.2 is prepared by using vegetable peptones in place of animal based peptones that makes the medium free of BSE/TSE risks. Acetobacter HiVeg Agar with HiVeg Extract No.2 is a modification of Acetobacter Agar with Liver Extract. Acetobacter Agar is cited in Manual of Microbiology Methods (1). The medium recommended is essentially adopted by the American Type Culture Collection, meant for maintenance and preservation of Acetobacter culture. Necessary carbon and nitrogen sources are provided by the ingredients of the medium viz. glucose as carbon and energy source whereas yeast extract and HiVeg extract No.2 serves as a source of nitrogen and essential growth requirements.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Product Profile
Vegetable based (Code MV)
MV346
- HiVeg hydrolysate
- HiVeg extract No. 2
Animal based (Code M)
M346
- Casein enzymic hydrolysate
- Liver extract
Recommended for
Use as a maintenance medium for glucose positive Acetobacter species.
Reconstitution
| Reconstitution | 57.0 g/l |
| Quantity on preparation (500g) | 8.77 L |
| pH (25°C) | 7.4 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity
Light amber coloured, clear to slightly opalescent gel forms in tubes.
Reaction
Reaction of 5.7% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Acetobacter aceti (15973) | luxuriant |
| Acetobacter liquefaciens (14835) | luxuriant |
| Product Name | Acetobacter HiVeg™ Agar w/ HiVeg™ Extract No. 2 |
|---|---|
| SKU | MV346 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Asai, 1968, Univ. of Tokyo Press, Tokyo, Japan and Univ. Park Press, Baltimore, MD 2.Catalogue of Bacteria and Bacteriophages, 1992, 18th Ed., American Type Culture Collection, Rockville, MD 3.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 4.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. 5.Manual of Microbiological Methods, 1957, Society of American Bacteriologists, McGraw-Hill Book Company, New York. 6.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C. |
| Customized Product Available | No |
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