Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective isolation and enumeration of all types of Streptococci including group A B-haemolytic strains.
Composition
| Ingredients | g / L |
|---|---|
| HiVeg™ hydrolysate | 15.000 |
| Soya peptone | 5.000 |
| Dextrose (Glucose) | 5.000 |
| Sodium chloride | 4.000 |
| Sodium citrate | 1.000 |
| Sodium sulphite | 0.200 |
| L-Cystine | 0.200 |
| Sodium azide | 0.200 |
| Crystal violet | 0.0002 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 45.6 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Autoclaving is not required if medium is used on the same day. If storage is desired, sterilize by autoclaving at A 118°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. A corresponds to 12 lbs pressure.
Principle And Interpretation
Streptococcus Selection Agar / Broth is formulated as per Pike (1), for the selective isolation of Streptococci from various materials, especially those which are heavily contaminated with accompanying heterogenous microbial flora (2). Abilities of these media to recover group A B-haemolytic Streptococci has been reported by Welch et al (3). Streptococcus Selection HiVeg™ Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate, Soya peptone, dextrose and salts in the medium provide nutrients essential for the growth of Streptococci. Sodium azide and sodium sulphite inhibit gram-negative rods while crystal violet suppresses Staphylococci. However, Streptococci are not affected by these inhibitors at these concentrations. Due to this reason, this media is useful in studies of streptococcal flora from nutritional, dental and epidemiological specimens. Growth of coliforms, Proteus, Pseudomonas and Bacillus species is markedly suppressed in this medium. However, some strains of Staphylococci and Pneumococci may grow in this medium. All streptococcal colonies must be confirmed for identification.
Type of specimen
Water samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards. (1) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- All presumptive streptococci must be confirmed for identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light to medium amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.56% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) |
Growth | Recovery |
|---|---|---|---|
| #Bacillus spizizenii ATCC 6633 (00003*) |
>=104 | inhibited | 0% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | >=50% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor | <=10% |
| Pseudomonas aeruginosa ATCC 27853(00025*) |
>=104 | inhibited | 0% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) |
50-100 | none-poor | <=10% |
| Streptococcus pyogenes ATCC 19615 |
50-100 | luxuriant | >=50% |
Key: *Corresponding WDCM numbers # Formerly known as Bacillus subtilis subsp. spizizenii.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Streptococcus Selection HiVeg™ Agar |
|---|---|
| SKU | MV304 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Pike R. M., 1945, Am. J. Hyg., 41:2 2.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. ,, 3.Welch D. F., Henel D., Pickett D., Johnson S., 1991, Am. J. Clin. Pathol., 95:587. |
| Customized Product Available | No |
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