Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for sensitivity tests with sulphonamides and other antimicrobial agents.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone No. 3 | 10.000 |
| HiVeg™ infusion | 10.000 |
| Dextrose (Glucose) | 10.000 |
| Sodium chloride | 3.000 |
| Disodium hydrogen phosphate | 2.000 |
| Sodium acetate | 1.000 |
| Adenine sulphate | 0.010 |
| Guanine | 0.010 |
| Uracil | 0.010 |
| Xanthine | 0.010 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 51.04 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at A 118-121°C for 15 minutes. Cool to 45-50°C and add sterile serum or blood aseptically if desired. Mix well and pour into sterile Petri plates. A corresponds to 12-15 lbs pressure respectively.
Principle And Interpretation
Sensitivity testing has been used for several decades as a guide for antimicrobial therapy of serious infections. Such testing is most frequently performed when bactericidal antimicrobial agent therapy is considered necessary. It has also been used to ensure that the infecting organism is killed by (not tolerant to) the bactericidal compounds. Sensitivity Test Medium is designed for use in sensitivity tests with sulphonamides and other antimicrobial agents (1).
Sensitivity Test HiVeg™ Medium is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones.
Incorporation of sodium acetate and HiVeg™ infusion in this medium renders the medium to give better defined zones of inhibition in sensitivity plate tests. HiVegтм peptone No. 3 supplies the nitrogenous nutrients to the organisms. Addition of nucleoside bases supports the growth of common gram-positive and gram-negative organisms. Dextrose serves as the carbohydrate and energy source for many microorganisms. The medium is well buffered and isotonic due to the inclusion of disodium phosphate and sodium chloride respectively.
Type of specimen
Isolated Microorganism from clinical sample
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Well isolated colonies must be used.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 5.1% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH: 7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | good-luxuriant | >=70% |
| $Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | good-luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant | >=70% |
Key: *Corresponding WDCM numbers $ Formerly known as Bacillus subtilis subsp spizizenii
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Sensitivity Test HiVeg™ Medium |
|---|---|
| SKU | MV296 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R.M., 1993, Handbook of Microbiological Media, CRC Press, Inc., Boca Raton. |
| Customized Product Available | No |
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