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Intended Use
Nutrient HiVeg medias are recommended as general purpose culture media for nonfastidious organisms and with addition of blood can be used for cultivating fastidious organisms.
Composition
| Ingredients | MV012 Grams/Litre | MV244 Grams/Litre |
|---|---|---|
| HiVeg peptone | 10.0 | 10.0 |
| HiVeg extract | 5.0 | 10.0 |
| Sodium chloride | 5.0 | 5.0 |
| Agar | 15.0 | - |
Final pH (at 25°C) 7.4 ± 0.2
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 35 grams of MV012 or 25 grams of MV244 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
These media are prepared by completely replacing animal based peptones with vegetable peptones, which makes the media free of BSE/TSE risks. Peptic digest of animal tissue and Beef extract are replaced with plant based HiVeg peptone and HiVeg extract respectively which are nutritionally rich and supply essential nitrogeneous compounds and growth factors (1).
Nutrient HiVeg Agar / Broth with 1% HiVeg peptone has almost double concentration of these nitrogen sources making it more nutritive. HiVeg extract and HiVeg peptone provides the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients to the nonfastidious organisms like Bacillus subtilis and Staphylococcus aureus. Sodium chloride maintains osmotic equilibrium of the medium.
With the addition of 10% v/v blood or other biological fluids like ascitic fluid, serum etc. these media are recommended for growing fastidious organisms.
Nutrient HiVeg Broth with 1% HiVeg peptone can be used as a sterility testing medium for aerobes against Nutrient Broth, (animal based) recommended for microbia limit tests as per standard pharmacopoeia (2).
Quality Control
Appearance of powder
Light yellow to yellow coloured may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV012.
Product Details
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV012/MV244 | M012/M244 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Recommended for: Use as general purpose culture media for nonfastidious organisms and growth of fastidious organisms on supplementation with blood.
| Reconstitution | (MV012): 35.0 g/l |
| (MV244): 25.0 g/l | |
| Quantity on preparation (500g) | (MV012): 14.28 L |
| (MV244): 20.0 L | |
| Quantity on preparation (100g) | (MV012): 2.85 L |
| (MV244): 4.0 L | |
| pH (25°C) | 7.4 ± 0.2 |
| Supplement | Blood, if desired |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Colour and Clarity
Light yellow coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes. With the addition of blood, cherry red coloured, opaque gel forms in petri plates.
Reaction
Reaction of 3.5% w/v of MV012 or 2.5% w/v of MV244 aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth in MV244 | Growth* on MV012 | Recovery on MV012* | Growth** on MV012 | Haemolysis on MV012** |
|---|---|---|---|---|---|---|
| Staphylococcus aureus (25923) | 102-103 | luxuriant | luxuriant | >70% | luxuriant | beta |
| Neisseria meningitidis (13090) | 102-103 | good | good | >50% | luxuriant | none |
| Streptococcus pneumoniae (6303) | 102-103 | good | good | >50% | luxuriant | alpha |
| Streptococcus pyogenes (19615) | 102-103 | good | good | >50% | luxuriant | beta |
Key: * = without blood, ** = with blood
References
- MacFaddin, J. (1985); Methods for Isolation- Cultivation- Identification- Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
- IP: Indian Pharmacopoeia, 1996. Govt. of India 1996. The controller of Publication, Delhi.
| Product Name | Nutrient HiVeg™ Broth w/ 1% HiVeg™ Peptone |
|---|---|
| SKU | MV244 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C. 2.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 3.Baird R.B ., Eaton A.D., and Rice E.W. (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater, 23rd ed., APHA, Washington, D.C. 4.IP: Indian Pharmacopoeia, 2018, Govt. of India, 201 |
| Customized Product Available | No |
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