Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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BHI HiVeg™ Agar w/PABA (Brain Heart Infusion w/ PABA and Agar, HiVeg™)
Used for culturing blood from patients under Sulphonamide therapy. The addition of agar improves growth of anaerobes.
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Intended Use
Recommended for improved growth of pathogens from blood from patients undergoing sulphonamide treatment.
Composition
| Ingredients | g / L |
|---|---|
| HiVeg™™ infusion | 10.000 |
| HiVeg™™ special infusion | 7.50 |
| HiVeg™™ peptone | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| p-Amino benzoic acid (PABA) | 0.050 |
| Agar | 1.000 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 38.05 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
BHI HiVeg™™ Agar w/PABA is highly nutritious media which can support luxuriant growth of wide variety of microorganisms including bacteria, yeasts and moulds (1) and is often used for isolation of pathogens from clinical specimens especially blood (2). Para amino benzoic acid is an active inhibitor of the bacteriostasis produced by the sulfonamide drugs; also it serves as an accessory growth factor for several species of bacteria (3). Therefore para amino benzoic acid incorporated in the medium helps to neutralize the effect of antimicrobials present in the blood of patients under sulphonamide therapy making isolation of organisms from blood easier. Agar in the medium reduces the oxygen tension and favors growth of facultative and obligatory anaerobic microorganisms. BHI HiVeg™™ Agar w/PABA is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™™ peptone, HiVeg™™ special infusion and HiVeg™™ infusion provides carbon, nitrogen, amino acids and vitamins. Dextrose serves as a source of energy. Sodium chloride helps in maintaining the osmotic equilibrium.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical tests must be carried out for complete identification.
Performance and Evaluation
Performace of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured, clear to very slightly opalescent solution without any precipitate
Reaction
Reaction of 3.8% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed with added 0.5 grams of sulphadiazine per litre after an incubation i)Bacteria at 35-37° C for 18-24 hours ii) Fungal at 25-30°C for 24-48 hours iii) Bacteroides species anaerobically for 18-48 hours .
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bacteroides fragilis ATCC 25285 | 50-100 | good-luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | BHI HiVeg™ Agar w/PABA (Brain Heart Infusion w/ PABA and Agar, HiVeg™) |
|---|---|
| SKU | MV213 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. MacFaddin J. F., 1985, Media for the Isolation-Cultivation-Identification- Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore 2.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th (Eds.), 2003, Manual of ClinicalMicrobiology, ASM, Washington, D.C. 3.Mirick G. S., 1943, Exp. Med., 78:255 |
| Customized Product Available | No |
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