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Soyabean HiVeg™ Medium w/ BCP, Sterile Powder
This is a gamma irradiated sterile powder recommended for the evaluation of sterility in manufacturing process and also recommended for the cultivation of a wide variety of microorganisms. With the addition of carbohydrates it can be also used for the fermentation studies.
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Intended Use
Soyabean HiVeg Medium with BCP (sterile powder) is gamma irradiated sterile powder recommended for the evaluation of sterility in manufacturing process. It iis also used as a general purpose medium for the cultivation of a wide variety of microorganisms. With the addition of carbohydrates it can be also used for the fermentation studies.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiVeg hydrolysate | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dibasic potassium phosphate | 2.500 |
| Dextrose | 2.500 |
| Bromocresol purple | 0.010 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Sterile powder can be used directly for the evaluation of sterility in manufacturing process. For sterile liquid medium aseptically add 30.01 grams in 1000 ml sterile distilled water. Heat if necessary to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEAT. Excessive heating is detrimental. Dispense aseptically in sterile tubes or flasks as desired.
Principle And Interpretation
Soyabean HiVeg Medium is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi (1-4). It can also be used as a general, all purpose cultivation medium (1). When tested for the growth of organisms in presence of indicator like bromocresol purple, the colour of the medium changes from purple to yellow. With the addition of carbohydrates it can be also used for the fermentation studies of fastidious and non-fastidious organisms.
HiVeg hydrolysate and soya peptone provides necessary amino acids and other complex nitrogenous substances. Dextrose serves as an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH.
Quality Control
Appearance: Light yellow to greenish yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Purple coloured clear solution without any precipitate
Reaction: Reaction of 3.0% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH: 7.10-7.50
Sterility test: No growth is observed after 14 days for Bacteria at 30-35°C and for fungi at 20-25°C.
Growth Promotion Test
In accordance with the harmonized method of USP/EP.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours (Candida albicans incubated at 25-30°C for 2-7 days).
| Organism | Inoculum (CFU) | Growth | Colour of medium |
|---|---|---|---|
| Candida albicans ATCC 10231 | 50-100 | luxuriant | yellow |
| Staphylococcus aureus ATCC 25923 | 50-100 | good-luxuriant | yellow |
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant | yellow |
| Bacillus subtilis ATCC 6633 | 50-100 | good-luxuriant | yellow |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Soyabean HiVeg™ Medium w/ BCP, Sterile Powder |
|---|---|
| SKU | MV1655G |
| Product Type | HiVeg™ |
| Physical Form | Sterile Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.MacFaddin. 198 |
| Customized Product Available | No |
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