Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg peptone No. 1 | 18.000 |
| HiVeg hydrolysate | 6.000 |
| Yeast extract | 10.000 |
| Sodium pyruvate | 2.000 |
| Glucose | 2.000 |
| Magnesium glycerophosphate | 1.000 |
| Magnesium sulphate | 0.500 |
| Sodium chloride | 5.000 |
| Lithium chloride | 10.000 |
| Disodium hydrogen phosphate anhydrous | 2.500 |
| Chromogenic substrate | 0.050 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 36.02 grams in 460 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile contents of 1 vial of L. mono Enrichment Supplement I (FD214) and sterile rehydrated contents of L. mono Selective Supplement I (FD212), L. mono Selective Supplement II (FD213). Mix well and pour into sterile Petri plates.
Warning: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.
Principle And Interpretation
Listeria monocytogenes is a gram-positive foodborne human pathogen responsible for serious infections in pregnant women that may ultimately result in abortion, stillbirth, birth of a child with neonatal listeriosis and meningitis or primary bacteremia in adults and juveniles. The pathogenicity of Listeria ivanovii for humans is uncertain. Since L. monocytogenes and L. innocua have similar biochemical properties, they cannot be differentiated on traditional media (PALCAM, Oxford).
L. mono Differential HiVeg™ Agar Base is a slight modification of L. mono Differential Agar Base which is based on the formulation of Ottoviani and Agosti (1, 2) for the selective and differential isolation of Listeria monocytogenes from food and animal feeds. It is prepared by replacing animal based peptones with veg peptones which are free from BSE/TSE risks.
HiVeg Peptone No.1, HiVeg Hydrolyaste, yeast extract and sodium pyruvate provide essential growth nutrients and nitrogenous substances. Glucose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Phosphate buffers the medium. Lithium chloride and added selective supplements (FD212 and FD213) inhibit accompanying microflora and allow the growth of Listeria species. Listeria species hydrolyse the chromogenic substrate which produces green coloured colonies. Differentiation of Listeria monocytogenes from other Listeria species is based on phosphatidylinositol-specific phospholipase C (PIPLC) activity. Phospholipase C enzyme hydrolyses the purified substrate (FD214) added to the medium resulting in an opaque halo around Listeria monocytogenes colonies.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured, opalescent gel forms in Petri plates
Reaction
Reaction of 7.2% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed with added sterile L. mono Selective supplement I (FD212), L. mono Selective Supplement II (FD213) and L.mono Enrichment supplement I (FD214) after an incubation at 35 - 37°C for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony | PIPLC activity |
|---|---|---|---|---|---|
| Cultural Response | |||||
| Candida albicans ATCC 10231 | >=10³ | inhibited | 0% | ||
| Enterococcus faecalis ATCC 29212 | >=10³ | inhibited | 0% | ||
| Escherichia coli ATCC 25922 | >=10³ | inhibited | 0% | ||
| Listeria innocua ATCC 33090 | >=10³ | luxuriant | >=50% | greenish-blue | negative |
| Listeria grayi ATCC 19120 | 50-100 | luxuriant | >=50% | greenish-blue | negative |
| Listeria ivanovii ATCC 19119 | 50-100 | luxuriant | >=50% | greenish-blue | positive, opaque halo around the colony exhibiting phophatidylinositol specific phospholipase acivity |
| Listeria monocytogenes ATCC 19112 | 50-100 | luxuriant | >=50% | greenish-blue | positive, opaque halo around the colony exhibiting phosphatidylinositol specific phospholipase activity |
| Listeria seeligeri ATCC 35967 | 50-100 | luxuriant | >=50% | greenish-blue | negative |
| Listeria welshimeri ATCC 43549 | 50-100 | luxuriant | >=50% | greenish-blue | negative |
| Pseudomonas aeruginosa ATCC 27853 | >=10³ | inhibited | 0% | ||
Storage and Shelf Life
Store at 2-8°C. Use before expiry date on the label.
| Product Name | L.mono Differential HiVeg™ Agar Base |
|---|---|
| SKU | MV1540 |
| Customized Product Available | No |
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