LI Broth, HiVeg™, Liver Infusion Broth, HiVeg™

Intended Use

Recommended for cultivation of Brucella and other anaerobic organisms.

Composition

Ingredients	g/L
HiVeg™ infusion No. 1	500.000
HiVeg™ peptone No. 3	10.000
Sodium chloride	5.000
Final pH (at 25°C)	6.9±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 35.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle And Interpretation

Brucella, a gram-negative intracellular parasite causes epizootic abortions in animals and septicemic febrile illness or localized infection of bone, tissue or organ systems in humans (1,2). Tryptose Agar with 5% serum remains the media of choice for isolation of Brucella species. However the growth is highly enhanced when grown on Liver Infusion media. Half strength LI Broth, HiVeg™ can be used for the isolation of Entamoeba histolytica (3). LI Broth, HiVeg™ is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.

HiVeg™ infusion No. 1 and HiVeg™ peptone No. 3 provide the nitrogen, amino acids, vitamins and carbon sources which permit luxuriant growth of Brucella and other fastidious pathogens. Sodium chloride maintains the osmotic balance. The reducing substances present in liver tissue create an anaerobic environment, which satisfies the requirements of even fastidious anaerobes. Refer appropriate references for standard procedures (4,5,6). Brucella species are highly infectious and extreme care should be taken while handling the cultures.

Type of specimen

Please add specimens

Specimen Collection and Handling

After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :

Brucella species are highly infectious and extreme care should be taken while handling the cultures.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Light yellow to brownish yellow homogeneous free flowing powder

Colour and Clarity of prepared medium
Amber coloured clear solution in tubes

Reaction
Reaction of 3.5% w/v aqueous solution at 25°C. pH: 6.9±0.2

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.(Clostridium species incubated anarobically)

Organism	Inoculum (CFU)	Growth
Brucella melitensis ATCC 4309	50-100	luxuriant
Brucella suis ATCC 4314	50-100	luxuriant
Clostridium sporogenes ATCC 11437	50-100	luxuriant
Streptococcus mitis ATCC 9811	50-100	luxuriant

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,8).

More Information
Product Name	LI Broth, HiVeg™ (Liver Infusion Broth, HiVeg™)
SKU	MV153
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Moyer N. P. and Holcomb L. A., 1988, Brucellosis, p. 143-154, In Balows A., Hausler W. J., Jr. Ohashi M. and Turano A.(Eds.), Laboratory Diagnosis and Infectious Diseases: Principle and Practice, Vol. I., Springer-Verlag, New York. 2.Smith, L. D. and Fieht T. A., 1990, Pathogenesis of Brucella. Crit. Rev. Microbiol., 17: 209-230. 3.Cleveland L. R. and Sanders E. P., 1930, Arch. Protietenkd. 70:223. 4.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 5.Forbes B. A., Sahm A. S., and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc.,St. Louis, Mo. 6.Isenberg H. D., (Ed.), 1995, Clinical Microbiology Procedures Handbook, Vol. I, ASM, Washington, D.C.
Customized Product Available	No