Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for detection, isolation and enumeration of yeasts and moulds.
Composition
| Ingredients | g/L |
|---|---|
| Malt extract | 30.000 |
| HiVeg™ peptone No. 4 | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.0 grams in 1000 ml purified/distilled water and soak for 15 minutes. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 10 minutes. Cool to 45-50°C. Mix well before pouring into sterile Petri plates. AVOID OVERHEATING. If desired to adjust acidic pH use 10% Lactic Acid (FD095).
Principle And Interpretation
The laboratory diagnosis of fungal infection relies largely on direct as opposed to indirect methods. The use of malt and malt extracts for the propagation of yeasts and moulds is quite common. Reddish (1) described a culture medium prepared from malt extract that was a satisfactory substitute for wort. Malt Extract Medium is similar to the formula of Galloway and Burgess (2) used for the detection, isolation and enumeration of yeasts and moulds. Malt Extract HiVeg™ Agar Base is same as Malt Extract Agar Base except that the animal based peptones are completely replaced with vegetable peptones to avoid the BSE/TSE risks associated with animal peptones.
Malt extract provides an acidic environment and nutrients favorable for growth and metabolism of yeasts and moulds. HiVeg™ peptone No. 4 rapidly gives a luxuriant growth with typical morphology and pigmentation. For mycological count, it is advisable to adjust the reaction of medium more acidic with addition of 10% lactic acid. Antibiotics may be added as sterile solutions to the molten medium immediately before pouring into sterile Petri plates (3) in order to suppress bacterial growth.
Type of specimen
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Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- It is a general purpose medium which supports growth of bacterial and fungal cultures.
- Further biochemical tests must be carried out for further identification
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to beige homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 5.0% w/v aqueous solution at 25°C. pH: 5.4±0.2
pH: 5.20-5.60
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
| Organism | Growth | Inoculum (CFU) | Recovery |
|---|---|---|---|
| Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant | 50-100 | |
| Candida albicans ATCC 10231 (00054*) | luxuriant | 50-100 | >=70% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | luxuriant | 50-100 | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10- 30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Malt Extract HiVeg™ Agar Base |
|---|---|
| SKU | MV137 |
| Customized Product Available | No |
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10% Lactic acid solution (10 ml per vial)
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