Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Microbiological assay of Polymyxin B, colistin sulphate and colistimethate sodium.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 17.0 |
| Papaic digest of soyabean meal | 3.0 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 2.5 |
| Glucose | 2.5 |
| Polysorbate 80 | 10.0 |
| Agar | 15.0 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 55.0 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by completely replacing animal based peptone with vegetable peptone making the medium free of BSE/TSE risks. Modified Soyabean HiVeg Agar is the modification of Modified Soyabean Casein Digest Agar which is recommended as Medium-B by British Pharmacopoeia for microbiological assay of Polymyxin B, Colistin sulphate and Colistimethate sodium (1). The combination of HiVeg hydrolysate and Papaic digest of soyabean meal makes this media nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Glucose serves as immediate carbon source whereas dipotassium phosphate buffers the medium. Sodium chloride maintains the osmotic balance of media. Depending on the growth requirement, polysorbate 80 is included in the media which serves as fatty acid source.
Quality Control
Appearance of powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Growth Promoting Properties
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacillus subtilis (6633) | 10²-10³ | luxuriant | >70% |
| Candida albicans (10231) | 10²-10³ | luxuriant | >70% |
| Staphylococcus aureus (25923) | 10²-10³ | luxuriant | >70% |
| Streptococcus pyogenes (19615) | 10²-10³ | luxuriant | >70% |
Storage and Shelf Life
Dry Medium Below 30°C, Prepared Medium 2 - 8°C.
References
- The BritishS Pharmacopoeia., 2003, Volume IV, The Stationery Office Limited, UK.
| Product Name | Modified Soyabean HiVeg™ Agar |
|---|---|
| SKU | MV1329 |
| Customized Product Available | No |
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