Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Micro Vitamin Test HiVeg media are recommended for cultivation and maintenance of stock cultures of Lactobacilli used in microbiological assays of vitamins.
Composition
| Ingredients | MV132 Grams/Litre |
MV133 Grams/Litre |
|---|---|---|
| Yeast extract | 20.00 | 20.00 |
| HiVeg peptone | 5.00 | — |
| HiVeg peptone No.3 | — | 5.00 |
| Dextrose | 10.00 | 10.00 |
| Monopotassium phosphate | 2.00 | 2.00 |
| Polysorbate - 80 | 0.10 | 0.10 |
| Agar | 15.00 | — |
Final pH (at 25°C) 6.7 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 52.1 grams of MV132 or 37.1 grams of MV133 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
- Quantity on preparation (100g):
- (MV132): 1.91 L
- (MV133): 2.69 L
Principle and Interpretation
Micro Vitamin Test HiVeg media are the modifications of Micro Vitamin Test media which are used for carrying stock cultures of Lactobacilli and other test organisms used in microbiological assays (1). These media can be used for routine cultivation of Lactobacilli.
HiVeg peptone or HiVeg peptone No.3 and yeast extract provide essential nutrients such as nitrogen, sulphur, vitamins etc. for growth. Dextrose is the energy source. Polysorbate 80 is the fatty acid source.
Stock cultures are prepared by stab inoculation in triplicate. One is used for the preparation of stock cultures while others are used for inoculum preparation for assays. Transfer of cultures should be made at weekly or biweekly intervals.
Quality Control
Appearance of Powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel of MV132.
Colour and Clarity: Light yellow coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction: Reaction of 5.2% w/v of MV132 or 3.71% w/v of MV133 aqueous solution is pH 6.7 ± 0.2 at 25°C.
Supplement: None
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 24-48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Lactobacillus leichmannii (7830) | good-luxuriant |
| Lactobacillus plantarum (8014) | good-luxuriant |
| Lactobacillus viridescens (4797) | good-luxuriant |
| Lactobacillus casei (9595) | good-luxuriant |
Storage and Shelf Life
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
| Product Name | Micro Vitamin Test Culture HiVeg™ Agar |
|---|---|
| SKU | MV132 |
| Customized Product Available | No |
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