Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| Part A: HiVeg peptone | 10.00 |
| Agar | 15.0 |
| Part B: Polysorbate 80 | 10.0 |
Final pH (at 25°C) 7.2 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 25.0 grams of Part A in 990 ml distilled water. Heat to boiling to dissolve the medium completely. Add 10 ml of Part B. Mix well and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Polysorbate 80 HiVeg Agar is prepared by using HiVeg peptone in place of peptic digest of animal tissue which is free from BSE/TSE risks. This medium is the modification of Polysorbate 80 Agar which is recommended for the cultivation of variety of microorganisms. It is a nutritional medium containing neutralizing agent. The medium contains HiVeg peptone which provide the necessary nutrients for the growth of the organisms. Polysorbate 80 provides fatty acids for the metabolism of the organisms and neutralizes phenolic disinfectants, hexachlorophene and formalin (1).
Product Specifications
Product Profile
| Vegetable based (Code MV) | |
|---|---|
| MV1307 | HiVeg peptone |
| Animal based (Code M) | |
|---|---|
| M1307 | Peptic digest of animal tissue |
Recommended for : Cultivation of variety of microorganisms.
Reconstitution : 25.0 g/lt of Part A + 10 ml/lt of Part B
Quantity on preparation (500g): 20.0 L (Part A)
pH (25°C) : 7.2 ± 0.2
Supplement : None
Sterilization : 121°C / 15 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
- Part A: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder
- Part B: Colourless, clear viscous liquid
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Yellow coloured, clear to slightly opalescent gel forms in petriplates.
Reaction
Reaction of the medium (2.5% w/v part A + 1.0% v/v part B) is pH 7.2 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacillus subtilis (6633) | 102-103 | luxuriant | >70% |
| Candida albicans (10231) | 102-103 | luxuriant | >70% |
| Escherichia coli (25922) | 102-103 | luxuriant | >70% |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% |
References
- Favero (chm.) 1967, Microbiological sampling of surfaces, Biological Contamination Control Committee, American Asso. For Contamination Control.
| Product Name | Polysorbate 80 HiVeg™ Agar (Twin Pack) |
|---|---|
| SKU | MV1307 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R. M., 1997, ln: Handbook of Microbiological Media, 2nd Edition, Lawrence C Parks (Ed.), CRC Press, London. |
| Customized Product Available | No |
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Polysorbate 80 Agar (Twin Pack)
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₹5,440.00