Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| HiVeg peptone | 1.000 |
| HiVeg extract no. 1 | 1.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 22 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Nutrient HiVeg Agar for Oxidase is prepared by completely replacing animal based peptones with veg peptones. This medium is the modification of Nutrient Agar recommended by APHA (1) for differentiation of the coliform bacteria on the basis of presence of enzyme cytochrome oxidase. Cytochrome oxidase is a iron-containing porphyrin enzyme that participates in the electron transfer mechanisms and in the nitrate metabolic pathways of some bacteria.
HiVeg peptone and HiVeg extract no. 1 provide nitrogenous compounds, carbon, sulphur and trace ingredients. Sodium chloride maintains osmotic equilibrium.
Nutrient HiVeg Agar plates are streak inoculated to obtain isolated colonies. The isolated colony is used for oxidase testing on an impregnated filter paper. A dark purple colour that develops within 10 seconds is a positive oxidase test. Coliform bacteria are oxidase negative.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 2.2% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH: 7.10-7.50
Cultural Response
MV1274: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Growth | Oxidase |
|---|---|---|
| Aeromonas hydrophila ATCC 7966 | luxuriant | positive reaction, deep purple blue colour develops within 10 seconds |
| Escherichia coli ATCC 25922 | luxuriant | negative reaction |
| Enterobacter aerogenes ATCC 13048 | luxuriant | negative reaction |
| Pseudomonas aeruginosa ATCC 27853 | luxuriant | positive reaction, deep purple blue colour develops within 10 seconds |
| Vibrio cholerae ATCC 15748 | luxuriant | positive reaction, deep purple blue colour develops within 10 seconds |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Nutrient HiVeg™ Agar for Oxidase |
|---|---|
| SKU | MV1274 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Greenberg A. E., Clesceri L. S. and Eaton A. D., (Eds.), 1992, Standard Methods for the Examination of Water andWastewater, 18th Ed., APHA, Maryland. |
| Customized Product Available | No |
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Nutrient Agar for Oxidase
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₹5,646.00