Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Tryptone Yeast Extract HiVeg Agar is recommended for estimation of microbial counts in water. The broth medium is used as a general purpose enrichment medium for cultivation of not particularly fastidious microorganisms.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
| MV1272/MV356 | M1272/M356 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Recommended for
Estimation of microbial counts in water and as a general purpose enrichment medium for cultivation of not particularly fastidious microorganisms.
Composition
| Ingredients | MV1272 Grams/Litre | MV356 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 6.0 | 5.0 |
| Yeast extract powder | 3.0 | 3.0 |
| Agar | 12.0 | - |
| Final pH (at 25°C) | 7.2 ± 0.2 | 7.0 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Reconstitution
(MV1272): 21.0 g/l
(MV356): 8.0 g/l
Quantity on preparation
(500g):
(MV1272): 23.80 L
(MV356): 62.50 L
(100g):
(MV356): 12.50 L
pH (25°C)
(MV1272): 7.2 ± 0.2
(MV356): 7.0 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 21 grams of MV1272 and 8 grams of MV356 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.
Principle and Interpretation
These media are prepared by using HiVeg hydrolysate which is free of BSE/TSE risks associated with animal based peptones. Tryptone Yeast Extract HiVeg Agar is the modification of Tryptone Yeast Extract HiVeg Agar which is formulated for the enumeration of viable microorganisms in water. Tryptone Yeast Extract HiVeg Broth is the modification of Tryptone Yeast Extract Broth formulated as per International Streptomyces Project (1).
HiVeg hydrolysate and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients.
For colony count, water sample to be tested is either streak inoculated on agar or is added in sterile petri plates and sterile molten agar is added to it and mixed with rotation and allowed to set. Tryptone Yeast Extract HiVeg Broth with added carbohydrate and a pH indicator, can be used for fermentation studies.
Quality Control
Appearance
Light yellow to yellow coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.2% Agar gel, of MV1272.
Colour and clarity
Light amber coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction
Reaction of 2.1% w/v aqueous solution of MV1272 is pH 7.2±0.2 at 25°C.
Reaction of 0.8% w/v aqueous solution of MV356 is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours on MV1272.
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 102-103 | luxuriant | >70% |
| Enterobacter aerogenes (13078) | 102-103 | luxuriant | >70% |
| Salmonella serotype Typhimurium (14028) | 102-103 | luxuriant | >70% |
| Salmonella serotype Enteritidis (13076) | 102-103 | luxuriant | >70% |
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours in MV356.
Organisms (ATCC)
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli (25922) | luxuriant |
| Staphylococcus aureus (25923) | luxuriant |
| Strptomyces albus (3004) | luxuriant |
| Streptomyces lavendulae (8664) | luxuriant |
References
- Shirling E.B. and Gotlieb D., 1966, International J. Systemic Bact, 16: 3.
| Product Name | Tryptone Yeast Extract HiVeg™ Agar |
|---|---|
| SKU | MV1272 |
| Customized Product Available | No |
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