Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
This medium is prepared by completely replacing animal based peptones with vegetable peptones. Recommended for confirmation of Listeria in Henry's light.
Composition**
ISO specification - Tryptone Soya Yeast Extract Broth
| Ingredients | g/L |
|---|---|
| Enzymatic digest of casein | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Dextrose (Glucose) | 2.500 |
| Yeast extract | 6.000 |
| Final pH (at 25°C) | 7.3±0.2 |
MV1263 - Tryptone Soya Yeast Extract HiVeg® Broth
| Ingredients | g/L |
|---|---|
| HiVeg® hydrolysate $ | 17.000 |
| Soya peptone# | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Dextrose (Glucose) | 2.500 |
| Yeast extract | 6.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Key $ - Equivalent to Enzymatic digest of casein, # - Equivalent to Papaic digest of soyabean meal
Directions
Suspend 36 gram in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Tryptone Soya Yeast Extract Broth is formulated as per APHA (1) for the isolation and cultivation of Listeria monocytogenes from foods. ISO Committee (2-4) .Tryptone Soya Yeast Extract HiVeg® Broth is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.
HiVeg® hydrolysate and soya peptone provide nitrogeneous and carbonaceous compounds, long chain amino acids and other essential nutrients. Dextrose is the energy source. Dipotassium hydrogen phosphate acts as buffering system to control pH. Yeast extract is the rich source of vitamin B complex.
Type of specimen
Food and animal feeds, environmental samples
Specimen Collection and Handling:
For food and animal feeds, environmental samples follow appropriate techniques for handling specimens as per established guidelines (1-4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further confirmation of organisms on selective media is required.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellow coloured clear solution in tubes.
Reaction
Reaction of 3.6% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Productivity: Cultural characteristics observed after an incubation at 25 ± 1°C for 21 ± 3 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Listeria monocytogenes ATCC 13932 (00021*) | 50-100 | good-luxuriant |
| Listeria monocytogenes ATCC 35152 (00109*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Tryptone Soya Yeast Extract HiVeg™ Broth |
|---|---|
| SKU | MV1263 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination ofFoods, 3rd Ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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Tryptone Soya Yeast Extract Broth
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