Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 50.0 |
| Papaic digest of soyabean meal | 5.0 |
| Sodium chloride | 30.0 |
| Magnesium sulphate | 1.5 |
| Agar | 15.0 |
Final pH (at 25°C) 7.3 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 10.15 grams in 100 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate which is free of BSE/TSE risks associated with animal based Casein enzymic hydrolysate in conventional Tryptone Soya Salt Agar with Magnesium Sulphate (TSAMS) (M1242) recommended by APHA for isolation of Vibrio parahaemolyticus from sea foods (1). The equivalent HiVeg media (MV1242) can be used for the same purpose. High level of sodium chloride, 3.0% concentration is used in this media which selectively allows growth of Vibrio species. Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio fluvialis is reported to survive at 8% concentration of sodium chloride whereas Vibrio vulnificus tolerates 6% of sodium chloride in growth media (1).
This medium has growth supporting nutrients favourable for Vibrio species. The sample can also be diluted and plated for enumeration if pure culture is used. However further confirmation needs to be studied on differential medium. As per APHA, initial use of equivalent media, TSAMS after membrane filtration and impinging on suitable agar media like Vibrio parhaemolyticus Sucrose Agar (VPSA) (M1153), for growth of Vibrio parahaemolyticus is cited. Utilizing hydrophobic grid membrane filter (HGMF), this medium can be used for enumeration of Vibrio parhaemolyticus as recommended by APHA (1,2). On similar lines, the suspected seafood samples may be diluted in suitable diluent (PTS- Peptone Tween Salt Diluent) and filtered through membrane. The membrane may then be aseptically transferred on MV1242 and incubated at 35-37°C for 4 hours. After incubation this membrane is transferred on dry Vibrio parahaemolyticus Sucrose HiVeg Agar (MV1153). Vibrio parahaemolyticus colonies appear as green to blue colonies while other Vibrios appear yellow due to fermentation of sucrose.
Please note that Vibrio vulnificus also grows as green to blue coloured colony on MV1153. It can be differentiated from Vibrio parahaemolyticus on basis of additional biochemical reactions. Vibrio parahaemolyticus does not ferment lactose, cellobiose and is ONPG negative whereas Vibrio vulnificus is positive for all these three tests.
Product Profile
| Vegetable based (Code MV)◉ | Animal based (Code M) | ||
| MV1242 | HiVeg hydrolysate | M1242 | Casein enzymic hydrolysate |
Recommended for
Enumeration of Vibrio parahaemolyticus from seafood by membrane filter technique.
Reconstitution Data
- Reconstitution
- 101.50 g/l
- Quantity on preparation (500g)
- 4.92 L
- pH (25°C)
- 7.3 ± 0.2
- Supplement
- None
- Sterilization
- 121°C / 15 minutes.
- Storage
- Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
Light yellow to yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 10.15% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 42°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Vibrio alginolyticus (17749) | 102-103 | good-luxuriant |
| Vibrio parahaemolyticus (17802) | 102-103 | good-luxuriant |
| Vibrio vulnificus (29306) | 102-103 | good-luxuriant |
References
- Downes FP and Ito K (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed., APHA, Washington, D.C.
- Entis, P. and Boleszcuk, P. 1983, J. Food Prot., 46:783.
| Product Name | Tryptone Soya Salt HiVeg™ Agar w/ Magnesium Sulphate |
|---|---|
| SKU | MV1242 |
| Customized Product Available | No |
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