Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Peptone Sorbitol HiVeg Broth is used for identification of Yersinia enterocolitica from dairy products.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.0 |
| Sorbitol | 10.0 |
| Disodium phosphate | 8.23 |
| Monosodium phosphate | 1.2 |
| Synthetic detergent No. I | 1.5 |
| Sodium chloride | 5.0 |
| Final pH (at 25°C) | 7.6 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Technical Data
| Recommended for | Identification of Yersinia enterocolitica from dairy products. |
| Reconstitution | 30.93 g/l |
| Quantity on preparation (500g) | 16.16 L |
| pH (25°C) | 7.6 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 30.93 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense 100 ml into Wheaton bottles. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Peptone Sorbitol HiVeg Broth is prepared by using HiVeg peptone and synthetic detergent No. I in place of Peptic digest of animal tissue and bile salts mixture. Peptone Sorbitol HiVeg Broth is the modification of Peptone Sorbitol Broth which is formulated as per APHA (1) for the identification of Yersinia enterocolitica from dairy products. Yersinia enterocolitica is a food borne pathogen which causes gastroenteritis and terminalileitis in humans. Peptone Sorbitol HiVeg Broth contains HiVeg peptone which provides nitrogenous growth factors and trace ingredients for the growth of Yersinia enterocolitica. Sorbitol is polyhydric alcohol corresponding to glucose which is an important substrate in biochemical characterization tests for bacteria. Yersinia enterocolitica can degrade sorbitol while Yersinia pestis and Yersinia pseudotuberculosis can not utilize it (1). Phosphates maintain the buffering action of the medium while synthetic detergent No. I inhibits majority of the gram-positive organisms. The pH of the medium is slightly alkaline as Yersinia species are tolerant to dilute alkali (2).
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Light yellow coloured, clear solution without any precipitate.
Reaction
Reaction of 3.09% w/v aqueous solution is pH 7.6 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 25°-30°C for 24-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Sorbitol utilization |
|---|---|---|---|
| Yersinia enterocolitica (27729) | 102-103 | luxuriant | + |
| Yersinia pseudotuberculosis (29833) | 102-103 | good-luxuriant | - |
References
- Standard Methods for the Examination of Dairy Products. 17th Edition, 2004 Edited by H. Michael Wehr and Joseph H.Frank.
- Aulisio C.C.G., Mehlman I.J. and Sanders A.C., 1980, Appl. Environ. Microbiol., 39:135.
| Product Name | Peptone Sorbitol HiVeg™ Broth |
|---|---|
| SKU | MV1231 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Doyle M.P., Hugdahl M.B., Taylor S. L., 1981, Appl. Environ. Microbiol. 42:661-666 |
| Customized Product Available | No |
Write Your Own Review
Peptone Sorbitol Bile Broth
As low as
₹6,508.00
Upsell Products
Yersinia Selective Agar Plate
As low as
₹1,227.00