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Bromo Cresol Purple Azide HiVeg™ Broth

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MV1212
For the confirmation of the presence of faecal Streptococci in water and waste water.

Intended Use

BCP Azide HiVeg Broth is used for the confirmation of the presence of faecal Streptococci in water and wastewater.

Composition

Ingredients Grams/Litre
HiVeg hydrolysate 10.0
Yeast extract 10.0
D-Glucose 5.0
Sodium chloride 5.0
Dipotassium hydrogen phosphate 2.7
Potassium dihydrogen phosphate 2.7
Sodium azide 0.5
Bromo cresol purple 0.032
Final pH (at 25°C) 7.0 ± 0.2

** Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 36 grams in 1000 ml distilled water. Add 5 ml/l glycerol if desired. Dispense into test tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes.

Warning: Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.

Principle and Interpretation

Bromocresol Purple Azide HiVeg Broth is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks which can be used for confirming the presence of Enterococci, particularly in bacteriological analysis of water according to Hajna and Perry (1). This medium can be used for testing after preliminary testing of water sample in the Azide Dextrose HiVeg Broth (MV345) or equivalent Azide Dextrose Broth (M345), medium recommended for enumerating of feacal Streptococci by MPN technique as cited in APHA (2).

Bromocresol Purple Azide HiVeg Broth has dextrose (D-glucose) as fermentable carbon source and added bromocresol purple as an indicator. The colour change of the medium from purple to yellow indicates fermentation of dextrose (D-glucose) due to growth and subsequent acid production. According to Hajna Enterococcal dextrose fermentation is improved by the addition of glycerol (1). HiVeg hydrolysate and yeast extract supply nitrogenous compounds, sulphur, amino acids and trace ingredients. Sodium chloride maintains osmotic balance of the medium. Sodium azide inhibits the entire bacterial flora including those species which may have grown in the preliminary test media. Colour change to yellow with turbidity indicates and confirms growth of Enterococci.

Quality Control

Appearance of powder
Beige coloured, may have slightly greenish tinge, homogeneous, free flowing powder.

Colour and Clarity
Purple coloured, clear solution without any precipitate.

Reaction
Reaction of 3.6% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.

Technical Specifications

Parameter Value
Vegetable based (Code MV) MV1212 HiVeg hydrolysate
Animal based (Code M) M1212 Casein enzymic hydrolysate
Reconstitution 36.0 g/l
Quantity on preparation (500g) 13.88 L
pH (25°C) 7.0 ± 0.2
Supplement Glycerol, if desired
Sterilization 115°C / 15 minutes.
Storage Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 24 - 48 hours.

Organisms (ATCC) Inoculum (CFU) Growth Acid
Enterococcus faecalis (29212) 102-103 good-luxuriant +
Escherichia coli (25922) 102-103 inhibited -
Pseudomonas aeruginosa (27853) 102-103 inhibited -
Streptococcus agalactiae (13813) 102-103 none-poor -
Streptococcus pyogenes (19615) 102-103 none-poor -

Key : + = positive reaction, yellow colour
- = negative reaction, no colour change

References

  1. Hajna, A. A. and Perry, C.A. 1943, Am. J. Publ. Health, 33: 550.
  2. Eaton, A.D., Clesceri, L.S and Greenberg, A.E. (eds.) 2005, Standard methods for the examination of water and wastewater, 21st edition, APHA, Washington, DC.
More Information
Product Name Bromo Cresol Purple Azide HiVeg™ Broth
SKU MV1212
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1. Cabelli V. J., 1983, EPA-600/1-80-031, U. S. Environmental Protection Agency, Cincinnati, Ohio.2.Dufour A. P., 1984, EPA-600/1-84-004, U. S. Environmental Protection Agency, Cincinnati, Ohio.3.Hajna A.A. and Perry C.A., 1943, Am. J. Publ. Health, 33:550.4.Hajna A.A., 1951, Public Health Lab., 9:80-81.5.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C.6.Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, AcademicPress, London.7.MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins,Baltimore.
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