Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Liver Meat Infusion HiVeg Agar is recommended for the enumeration of sulphite reducing Clostridia and Clostridium perfringens in water and milk.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg infusion No.2 | 20.00 |
| Dextrose | 0.75 |
| Starch | 0.75 |
| Sodium sulphite | 1.20 |
| Ferric ammonium citrate | 0.50 |
| Sodium carbonate | 0.67 |
| Agar | 11.00 |
Final pH (at 25°C) 7.6 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1206 | M1206 |
| HiVeg infusion No.2 | Meat liver infusion powder |
Recommended for
The enumeration of sulphite reducing Clostridia & Clostridium perfringens in water and milk.
Reconstitution
| Reconstitution | : 34.87 g/l |
| Quantity on preparation (500g) | : 14.33 L |
| pH (25°C) | : 7.6 ± 0.2 |
| Supplement | : None |
| Sterilization | : 121°C / 15 minutes. |
| Storage | : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 34.87 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using vegetable peptones in place of animal based peptones which make the medium free of BSE/TSE risks. Presence of HiVeg infusion No.2 in the medium provides adequate degree of anaerobiosis besides provision of rich supply of nutrients, enabling even strict and fastidious anaerobes to grow well. Clostridium species reduce sulphite present in the medium to hydrogen sulphide (H₂S), which is indicated by blackening due to the presence of iron salt. The agar medium is inoculated either by pour plate method or by surface spreading methods.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.1% Agar gel.
Colour and Clarity
Light amber coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 3.4% w/v aqueous solution is pH 7.6 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37° C for 24-48 hours in an anaerobic environment.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | H₂S production |
|---|---|---|---|---|
| Clostridium perfringens (12924) | 10²-10³ | luxuriant | >70% | + |
| Clostridium tetani (10779) | 10²-10³ | luxuriant | >70% | + |
| Clostridium botulinum (25763) | 10²-10³ | luxuriant | >70% | + |
| Escherichia coli (25922) | 10²-10³ | luxuriant | >70% | — |
| Proteus mirabilis (25933) | 10²-10³ | luxuriant | >70% | —/weak |
| Bacteroides vulgatus (8482) | 10²-10³ | luxuriant | >70% | — |
Key : + = blackening of the medium
| Product Name | LM Infusion HiVeg™ Agar (Liver Meat Infusion HiVeg™ Agar) |
|---|---|
| SKU | MV1206 |
| Customized Product Available | No |
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