Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Tryptone Water Broth, HiVeg™ is used for the cultivation of Salmonella species from foods.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 10.0 |
| Dextrose | 5.0 |
| Dipotassium phosphate | 1.25 |
| Yeast extract | 1.0 |
| Bromo cresol purple | 0.04 |
| Final pH (at 25°C) 7.0 ± 0.2 | |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 17.29 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense 10 ml portions in the size 20 x 150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Tryptone Water Broth HiVeg is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptones. Tryptone Water Broth, HiVeg is the modification of Tryptone Water Broth which is recommended and prepared as per APHA (1) for cultivating Salmonella species from foods. HiVeg hydrolysate and yeast extract provide the essential nitrogenous compounds, vitamin B complex and other growth nutrients for the growth of Salmonellae. Dipotassium phosphate serves as a buffer thereby maintaining the pH. Dextrose is the fermentable carbohydrate source. Bromo cresol purple is the pH indicator. The medium changes to yellow colour on utilization of dextrose.
Technical Data
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV1198 | HiVeg hydrolysate | M1198 | Casein enzymic hydrolysate |
| Recommended for | Cultivation of Salmonella species from foods. |
| Reconstitution | 17.29 g/l |
| Quantity on preparation (500g) | 28.91 L |
| pH (25°C) | 7.0 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour change of Medium |
|---|---|---|---|
| Salmonella serotype Enteritidis (13076) | 102-103 | luxuriant | yellow |
| Salmonella serotype Typhimurium (14028) | 102-103 | luxuriant | yellow |
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Purple coloured, clear solution without any precipitate.
Reaction
Reaction of 1.73% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
References
- Vanderzant C. and Splittstoesser D. (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods, 3rd ed., APHA, Washington, D.C.
| Product Name | Tryptone Water Broth, HiVeg™ |
|---|---|
| SKU | MV1198 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C. |
| Customized Product Available | No |
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