Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV119 | M119 |
| HiVeg special peptone | Peptone special |
Recommended for
Detection of pyocyanin production by Pseudomonas aeruginosa.
Reconstitution
46.4 g/l
| Quantity on preparation (500g) | 10.77 L |
| (100g) | 2.15 L |
pH (25°C)
7.0 ± 0.2
Supplement
Glycerol
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg special peptone | 20.00 |
| Potassium sulphate | 10.00 |
| Magnesium chloride | 1.40 |
| Agar | 15.00 |
Final pH (at 25°C) 7.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 46.4 grams in 1000 ml distilled water containing 10 ml glycerol. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Pseudomonas HiVeg Agar is prepared by using HiVeg special peptone, which is free from BSE/TSE risks associated with animal based peptones. It is the modification of Pseudomonas Agar which is based on the formulation described by King et al (1) for detecting pyocyanin, a water soluble pigment by Pseudomonas species (2). This medium enhances the elaboration of pyocyanin but inhibits the formation of fluorescein pigment. The pyocyanin pigment diffuses from the colonies of Pseudomonas into the agar and shows blue colouration. Some Pseudomonas strains produce small amounts of fluorescein resulting in a blue-green colouration. HiVeg special peptone provides nitrogenous growth nutrients, carbon, sulphur and trace elements for Pseudomonas species. Potassium sulphate and magnesium chloride enhances the pyocyanin production and suppresses the fluorescein production. Glycerol acts as a source of energy and enhances pigment production.
Quality Control
Appearance of powder Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling Firm, comparable with 1.5% Agar gel.
Colour and Clarity Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction Reaction of 4.64% w/v aqueous solution (containing 1% v/v glycerol) is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum | Growth | Colour of Medium |
|---|---|---|---|
| Pseudomonas aeruginosa (27853) | 30-300 | luxuriant | blue-green |
References
- King, Ward and Raney, 1954, J. Lab. Clin. Med., 44: 301.
- MacFaddin J., 1985, Media for Isolation-Cultivation-Identification- Maintenance of Medical Bacteria, Volume I, Williams and Wilkins, Baltimore.
| Product Name | Pseudomonas HiVeg™ Agar (For Pyocyanin) |
|---|---|
| SKU | MV119 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.King, Ward and Raney, 1954, J.Lab. and Clin. Med., 44:3012.The United States Pharmacopoeia, 2008, The United States Pharmacopoeial Convention, Rockville, MD. 3.MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams andWilkins, Baltimore. |
| Customized Product Available | No |
Write Your Own Review
Pseudomonas Agar, Granulated (For Pyocyanin)
As low as
₹5,912.00
Pseudomonas Agar (For Pyocyanin) (In a accordance with IP 2007)
As low as
₹1,280.00
Pseudomonas Agar For Detection of Pyocyanin
As low as
₹0.00
Upsell Products
Pseudomonas Agar For Detection of Pyocyanin
As low as
₹0.00
Pseudomonas Pyocyanin Agar Plate
As low as
₹3,381.00