Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Modified MRS HiVeg Broth is recommended for the isolation and cultivation of Lactobacillus species from foods.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Dextrose | 20.000 |
| HiVeg peptone No. 3 | 10.000 |
| Yeast extract | 5.000 |
| Sodium acetate | 5.000 |
| 2-Phenylethyl alcohol | 3.000 |
| Ammonium citrate | 2.000 |
| Dipotassium phosphate | 2.000 |
| Magnesium sulphate | 0.100 |
| Manganese sulphate | 0.050 |
| Bromo cresol green | 0.040 |
| Cycloheximide | 0.004 |
| Final pH (at 25°C) | 4.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.20 grams in 1000 ml distilled water containing 1 ml polysorbate 80. Mix thoroughly and dispense in tubes containing inverted Durham's tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary, adjust the pH with glacial acetic acid after sterilization.
Warning: Cycloheximide is very toxic. Avoid skin contact or aerosol formation and inhalation.
Principle And Interpretation
MRS Media is the modification of MRS medium of deMan et al (1) recommended for isolation and cultivation of Lactobacilli causing spoilage of salad dressings (2, 3).
HiVeg peptone no. 3 and dextrose supply nitrogen, carbon and other elements essential for the growth of Lactobacilli. Polysorbate 80 a mixture of oleic esters, supplies fatty acids required by Lactobacilli. Ammonium citrate, sodium acetate, 2-phenylethyl alcohol and Cycloheximide inhibit gram-negative organisms, moulds and certain gram-positive bacteria. Certain yeasts are also suppressed because of the presence of Cycloheximide.
Inoculate 1ml of 1:10 dilutions of the dressing sample into three MRS HiVeg Broth tubes. Incubate at 32°C for 72 hours ±2 hours. Positive tubes have trapped CO2 in the Durham's tubes or bubbles of CO2 clinging to the inside of the tube and a colour change from green to yellow indicating acid production.
Quality Control
Appearance: Light blue to bluish grey homogeneous free flowing powder
Colour and Clarity of prepared medium: Green coloured clear to slightly opalescent solution in tubes
Reaction: Reaction of 4.72% w/v aqueous solution at 25°C. pH : 4.3±0.2
pH: 4.10-4.50
Cultural Response
Cultural characteristics observed in presence of 5-10% Carbon dioxide (CO2) after an incubation at 35-37°C for upto 3 days.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus acidophilus ATCC 4356 | 50-100 | luxuriant |
| Lactobacillus fermentum ATCC 9338 | 50-100 | luxuriant |
| Lactobacillus plantarum ATCC 8014 | 50-100 | luxuriant |
Storage and Shelf Life
Store dehydrated and the prepared medium at 2 - 8°C in tightly closed container. Use before expiry date on the label.
| Product Name | Lactobacillus Heteroferm Screen HiVeg™ Broth |
|---|---|
| SKU | MV1164 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods,3rd Ed., APHA, Washington, D.C.2.Smittle R. B. and Flowers R. M., 1982, J. Food Protection, 45:977.3.DeMan J. D., Rogosa M. and Sharpe M. E., 1960, J. Appl. Bacteriol.,23:130. |
| Customized Product Available | No |
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