Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
TMAO HiVeg™ Medium (Trimethylamine-N-Oxide HiVeg™ Medium)
For cultivation and differentiation of Campylobacter species from food, except Campylobacter jejuni and Campylobacter coli in accordance with APHA.
Your enquiry has been submitted
Please login to Post an Enquiry.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1159 | M1159 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Recommended for
Cultivation and differentiation of Campylobacter species from foods, except Campylobacter jejuni and Campylobacter coli.
Reconstitution Details
| Reconstitution | 29.0 g/l |
| Quantity on preparation (500g) | 17.24 L |
| pH (25°C) | 7.5 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium Below 30°C, Prepared Medium 2 - 8°C. |
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.0 |
| HiVeg extract | 10.0 |
| Sodium chloride | 5.0 |
| Yeast extract | 1.0 |
| Trimethylamine-N-Oxide | 1.0 |
| Agar | 2.0 |
| Final pH (at 25°C) | 7.5 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 29 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense 4 ml in 13 x 100 mm screw cap tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes in an upright position.
Principle and Interpretation
TMAO (Trimethylamine-N-Oxide) HiVeg Medium is prepared by using HiVeg peptone and HiVeg extract which are free from BSE/TSE risks associated with animal based peptones. TMAO (Trimethylamine-N-Oxide) HiVeg Medium is the modification of TMAO (Trimethylamine-N-Oxide) Medium which is prepared as recommended by APHA (1) for cultivation and differentiation of Campylobacter species from foods except Campylobacter jejuni and Campylobacter coli. Campylobacters are mainly present in the intestinal tract of animals and therefore contaminate the foods of animal origin. Campylobacter jejuni and Campylobacter coli are sensitive to Trimethylamine-N-Oxide and hence do not grow in this medium. Campylobacter lari grows in this medium as it is not sensitive to this compound.
HiVeg extract, HiVeg peptone and yeast extract provide nitrogenous compounds, vitamin B complex and growth factors for Campylobacter lari. Sodium chloride maintains the isotonic atmosphere in the medium.
Culture is stab inoculated in upper one third of the medium and incubated in anaerobic condition for 7 days with loose caps. Growth of Campylobacter lari can be observed away from the stab line.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Semisolid, comparable with 0.2% Agar gel.
Colour and Clarity
Yellow coloured, clear to slightly opalescent gel forms in tubes as butts.
Reaction
Reaction of 2.9% w/v aqueous solution is pH 7.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 42°C for 24 - 48 hours under anaerobic condition.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Campylobacter coli (33559) | 102-103 | inhibited |
| Campylobacter jejuni (29428) | 102-103 | inhibited |
| Campylobacter lari (35221) | 102-103 | *good-luxuriant |
Key : * = observed for growth upto to 7 days
| Product Name | TMAO HiVeg™ Medium (Trimethylamine-N-Oxide HiVeg™ Medium) |
|---|---|
| SKU | MV1159 |
| Customized Product Available | No |
Write Your Own Review