Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg hydrolysate | 2.500 |
| HiVeg peptone | 2.500 |
| Yeast extract | 20.000 |
| Dextrose | 10.000 |
| Potassium dihydrogen phosphate | 2.000 |
| Polysorbate 80 | 0.100 |
| Agar | 10.000 |
| Final pH (at 25°C) | 6.7±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.1 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Antibiotic HiVeg Assay Medium No. 40 is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE TSE risks free. This can be used for the same purpose of Antibiotic HiVeg Medium No. 40, This medium is used as a maintenance medium for test organism Enterococcus hirae ATCC 10541 ( Streptococcus faecium) used for in the assay of Thiostrepton. Equivalent animal based medium is in accordance with USP (1). Essential amino acids, mineral and growth factors are supplied by Peptone, HiVeg hydrolysate and yeast extract in this medium. Dextrose functions as carbon and energy source for enhancing the growth of test organism. During maintenance of the test organism, good buffering action is maintained by phosphates in the medium. Incorporation of polysorbates reduces the surface tension, maintaining uniform suspension of cells and also can neutralize phenolic compounds in the test sample, if any.
Note: For Antibiotic Assay Methods and Selection of Antibiotic HiVeg Assay Medias Refer Section Antibiotic HiVeg Assay Media.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.0% Agar gel.
Colour and Clarity of prepared medium
Light amber clear to slightly opalescent gel forms in petriplates
Reaction
Reaction of 4.71% w/v aqueous solution at 25°C. pH : 6.7±0.2
pH
6.50-6.90
Cultural Response
MV1143: Cultural characteristics observed after an incubation at 35 - 37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Enterococcus hirae ATCC 10541 | 50-100 | luxuriant | >70% | Thiostrepton |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on label.
| Product Name | Antibiotic HiVeg™ Assay Medium No. 40 |
|---|---|
| SKU | MV1143 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. United States Pharmacopoeia 2009,US Pharmacopoeial Convention, Inc., Rockville, MD. |
| Customized Product Available | No |
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Antibiotic Assay Medium No. 40
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