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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg peptone | 6.000 |
| HiVeg hydrolysate | 4.000 |
| Yeast extract | 3.000 |
| HiVeg extract | 1.500 |
| Dextrose | 1.000 |
| Manganese sulphate | 0.300 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.8 gms in 1000 ml purified/distilled water. Heat to boiling to disslove the medium completely. Sterilise by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Antibiotic HiVeg Assay Medium No. 32 is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE-TSE risks free. This can be used for the same purpose of Antibiotic Assay Medium No. 32 that is formulated in accordance to FDA (1) and is a modification of Antibiotic assay Medium No.1. This medium is used to develop inoculum of Bacillus subtilis for antibiotic assay.
Essential nutrients, vitamins, mineral, trace elements and growth factors are supplied by HiVeg Peptone, HiVeg hydrolysate, yeast extract and HiVeg extract. Dextrose in the medium serves as the carbon source for stimulating the growth of the test microorganism. Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis (2,3,4), which is generally used as test organisms for plate assay of Dihydrostreptomycin and Vancomycin.
Note: For Antibiotic Assay Methods and Selection of Antibiotic HiVeg Assay Medias Refer Section Antibiotic HiVeg Assay Media.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow clear to slightly opalescent gel forms in Petriplates
Reaction: Reaction of 3.08% w/v aqueous solution at 25°C. pH : 6.6±0.2
pH: 6.40-6.80
Cultural Response: MV1141: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | good-luxuriant | >=70% | Dihydrostreptomycin, Vancomycin |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic HiVeg™ Assay Medium No. 32 |
|---|---|
| SKU | MV1141 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983 Title 21, Part 436, SubpartD, Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242-259, (April1). ,, |
| Customized Product Available | No |
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