Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective isolation and differentiation of Salmonella and Shigella species.
Composition**
| Ingredients | g/L |
|---|---|
| HiVegâ„¢ peptone | 5.000 |
| HiVegâ„¢ extract | 5.000 |
| Lactose | 10.000 |
| Sodium citrate | 5.000 |
| Ferric citrate | 1.000 |
| Synthetic detergent No. III | 2.500 |
| Sodium thiosulphate | 5.000 |
| Neutral red | 0.025 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 48.52 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE OR REMELT. Excessive heating is detrimental. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Leifson Deoxycholate Agar, was originally described by Leifson (1) and further modified by Hynes (2) for selective isolation and differentiation of Salmonella and Shigella species. This medium is the modification of Leifson Agar for the isolation and maximum recovery of intestinal pathogens. Leifson Deoxycholate Agar, Modified is a less selective medium and is used for direct sampling of faeces. Leifson's Deoxycholate HiVegâ„¢ Agar, Modified is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVegâ„¢ peptone and HiVegâ„¢ extract provide nitrogeneous and carbonaceous compounds, long chain amino acids and other essential growth nutrients. Sodium citrate and Synthetic detergent No. III inhibit all gram-positive bacteria and coliforms but allow the gram-negative bacilli to grow. Lactose is added to the medium to allow differentiation of lactose fermenting bacteria such as, Escherichia coli from non-lactose fermenting species, such as Salmonella, Proteus and Shigella. Lactose fermenting strains grow as red to pink colonies because of absorption of neutral red indicator. Nonfermenting species grow as colourless colonies. Ferric citrate and sodium thiosulphate help in H2S determination.
Type of specimen
Food and dairy samples
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored atex recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Reddish orange coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.85% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 28-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor | <=10% | pink with zone of precipitation |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=50% | colourless - tan |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | >=50% | colourless, black centred colonies |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=50% | colourless, black centered colonies |
| Shigella sonnei ATCC 25931 | 50-100 | good-luxuriant | >=50% |
Key : *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Leifson’s Deoxycholate HiVeg™ Agar, Modified |
|---|---|
| SKU | MV1138 |
| Product Type | HiVegâ„¢ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Leifson E., 1935, J. Pathol. Bacteriol., 40:581.2.Hynes M., 1942, J. Pathol. Bacteriol., 40:581.3.MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams andWilkins, Baltimore. |
| Customized Product Available | No |
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