Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
M-FC HiVeg Agar Base / Broth Base is used for the detection and enumeration of faecal coliforms using membrane filter technique at higher temperature
Composition
| Ingredients | MV1122 Grams/Litre |
MV1111 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate No. 1 | 10.00 | 10.00 |
| HiVeg peptone No. 3 | 5.00 | 5.00 |
| Yeast extract | 3.00 | 3.00 |
| Lactose | 12.50 | 12.50 |
| Synthetic detergent No. I | 1.50 | 1.50 |
| Sodium chloride | 5.00 | 5.00 |
| Aniline blue | 0.10 | 0.10 |
| Agar | 15.00 | — |
| Final pH (at 25°C) | 7.4 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
MV1122/MV1111
- HiVeg hydrolysate No. 1
- HiVeg peptone No. 3
- Synthetic detergent No. I
Animal based (Code M)
M1122/M1111
- Tryptose
- Proteose peptone
- Bile salts mixture
Recommended for
Detection and enumeration of faecal coliforms using membrane filter technique at higher temperature.
Reconstitution
| (MV1122) | 52.1 g/l |
| (MV1111) | 37.1 g/l |
Quantity on preparation (500g)
| (MV1122) | 9.59 L |
| (MV1111) | 13.47 L |
pH (25°C)
7.4 ± 0.2
Supplement
Rosolic acid (FD058)
Sterilization
Boiling (DO NOT AUTOCLAVE)
Storage
Dry Medium - Below 30°C, Use freshly prepared medium.
Directions
Suspend 52.1 grams of MV1122 or 37.1 grams of MV1111 in 1000 ml distilled water containing 10 ml 1% Rosolic Acid (FD058). Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45°C and add 2 ml of M-FC Broth on sterile absorbent pad placed in a sterile petri plate.
Principle and Interpretation
M-FC HiVeg Agar Base/Broth Base is prepared by using vegetable peptones in place of animal based peptones which makes the media free of BSE/TSE risks. These media are the modifications of M-FC Agar Base/ Broth Base which were designed by Geldreich, Clark, Huff and Bert (1) and recommended by APHA (2) for the detection and enumeration of faecal coliforms using membrane filter technique. Faecal coliforms are differentiated from coliforms from environmental sources by their ability to grow at 44.5 + 0.5°C (2). Faecal coliforms give blue coloured colonies
HiVeg peptone No. 3, HiVeg hydrolysate No. 1 and yeast extract provide necessary nutrients for the growth of faecal coliforms. Lactose is the carbon source as well as fermentable carbohydrate in the medium. Synthetic detergent No. I inhibits the growth of contaminating gram-positive microorganisms. Aniline blue and Rosolic acid (FD058) are the differential indicators
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV1122.
Colour and Clarity
With addition of rosolic acid, red coloured slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction
Reaction of 5.21% w/v of MV1122 or 3.71% w/v of MV1111 aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation for 22 - 24 hours at ...
| Organisms (ATCC) |
Inoculum (CFU) |
at 35°C | at 45.5°C | |||
|---|---|---|---|---|---|---|
| Growth | Recovery | Colour of the colony* |
Growth | Recovery | ||
| Escherichia coli (25922) |
10-100 | luxuriant | >50% | light blue | luxuriant | >50% |
| Salmonella serotype Typhimurium (14028) |
10-100 | luxuriant | >50% | pinkish | inhibited | 0% |
| Shigella flexneri (12022) |
10-100 | luxuriant | >50% | pinkish | inhibited | 0% |
| Enterococcus faecalis (29212) |
103-2x103 | inhibited | 0% | — | inhibited | 0% |
Key: * = on membrane filter
| Product Name | M-FC HiVeg™ Agar Base |
|---|---|
| SKU | MV1122 |
| Customized Product Available | No |
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Rosolic Acid (0.1 gm, vl)
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₹1,988.00