Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
M-Endo HiVeg™ Broth MF (MF Endo HiVeg™ Medium) (M-Coliform HiVeg™ Broth)
For enumeration of coliform bacteria in water samples using one step membrane filtration technique.
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate No. 1 | 10.0 |
| HiVeg hydrolysate | 5.0 |
| HiVeg special peptone | 5.0 |
| Yeast extract | 1.5 |
| Lactose | 12.5 |
| Synthetic detergent No. III | 0.1 |
| Dipotassium phosphate | 4.375 |
| Monopotassium phosphate | 1.375 |
| Sodium chloride | 5.0 |
| Sodium lauryl sulphate | 0.05 |
| Sodium sulphite | 2.1 |
| Basic fuchsin | 1.05 |
Final pH (at 25°C) 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
| MV1103 |
| HiVeg hydrolysate No. 1 |
| HiVeg hydrolysate |
| HiVeg special peptone |
| Synthetic detergent No. III |
Animal based (Code M)
| M1103 |
| Tryptose |
| Casein enzymic hydrolysate |
| Peptone special |
| Sodium deoxycholate |
Recommended for
Enumeration of coliform bacteria in water by the membrane filter technique.
Reconstitution
| Quantity on preparation (500g): | 48.0 g/l |
| pH (25°C) | 7.2 ± 0.2 |
| Supplement | Ethanol |
| Sterilization | Boiling (DO NOT AUTOCLAVE) |
| Storage | Dry Medium - Below 30°C, Use freshly prepared Medium. |
Directions
Suspend 48 grams in 1000 ml distilled water containing 20 ml ethanol. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to room temperature and dispense about 2 ml onto sterile absorbent pads. This medium should be used on the same day it is prepared and should be protected from bright light.
Caution: Basic fuchsin is a potential carcinogen and care must be taken to avoid inhalation and contamination of the skin.
Principle and Interpretation
M-Endo HiVeg Broth MF is prepared by completely replacing animal based peptones with vegetable peptones. M-Endo HiVeg Broth MF is the modification of M-Endo Broth MF which is a selective and differential medium for the detection of coliforms by the membrane filter technique (1). Preliminary enrichment on a non-selective medium is not required in case of M-Endo HiVeg Broth MF and this is a medium of choice for the determination of coliform bacteria in water and other specimens. Essential nutrients are supplied by HiVeg hydrolysate No.1, yeast extract, HiVeg special peptone and HiVeg hydrolysate. They provide mainly nitrogenous nutrients. Synthetic detergent No. III inhibits gram-positive bacteria. Coliforms ferment lactose and form pink colonies with metallic sheen on this medium.
Quality Control
Appearance of powder Purple coloured, homogeneous, free flowing powder.
Colour and Clarity Pinkish red coloured, opalescent solution without any precipitate.
Reaction Reaction of 4.8% w/v aqueous solution containing 2.0% v/v ethanol is pH 7.2 ± 0.2 at 25°C.
Cultural Response Cultural characteristics observed after an incubation at 35-37°C for 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of colony* |
|---|---|---|---|
| Escherichia coli (25922) | 10-10² | luxuriant | pink with metallic sheen |
| Salmonella serotype Typhimurium (14028) | 10-10² | luxuriant | colourless to slightly pink |
| Staphylococcus aureus (25923) | 10-10² | luxuriant | - |
Key : * = on membrane filter
| Product Name | M-Endo HiVeg™ Broth MF (MF Endo HiVeg™ Medium) (M-Coliform HiVeg™ Broth) |
|---|---|
| SKU | MV1103 |
| Customized Product Available | No |
Write Your Own Review