Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Description
M-Bismuth Sulphite HiVeg Broth is a selective medium used for the detection of Salmonellae by the membrane filter technique.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 20.0 |
| HiVeg extract | 10.0 |
| Dextrose | 10.0 |
| Disodium phosphate | 8.0 |
| Ferrous sulphate | 0.6 |
| Bismuth sulphite indicator | 16.0 |
| Brilliant green | 0.05 |
| Final pH (at 25°C) | 7.7 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1101 | M1101 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Product Specifications
Recommended for: The detection of Salmonellae by the membrane filter technique
Reconstitution: 64.65 g/l
Quantity on preparation (500g): 7.73 L
pH (25°C): 7.7 ± 0.2
Supplement: None
Sterilization: Boiling (DO NOT AUTOCLAVE)
Storage: Dry Medium - Below 30°C, Use freshly prepared medium.
Directions
Suspend 64.65 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Excessive heating destroys the selective properties of the medium. DO NOT AUTOCLAVE. The medium usually contains flocculent precipitate which should be dispersed evenly by swirling the flask just before use. Cool to 35°C and saturate sterile absorbent cotton pad with 2 ml of the broth. The medium should be used within 24 hours of rehydration.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and HiVeg extract which are free from BSE/TSE risks associated with animal based peptones. M-Bismuth Sulphite HiVeg Broth is the modification of M-Bismuth Sulphite Broth which was formulated by Clark et al (1) and is particularly recommended for detection of Salmonella serotype Typhi from water and various clinical specimens. Preliminary enrichment on a nonselective medium is not necessary. HiVeg peptone, HiVeg extract and dextrose provides essential growth nutrients. Ferrous sulphate and bismuth sulphite indicator together act as H2S indicators. Brilliant green acts as selective agent. Luxuriant growth of Salmonella serotype Typhi is obtained after 30 hours incubation at 35°C but metallic sheen and brown-black halo is not developed before 40 hours. The importance of this medium like the conventional medium has been repeatedly mentioned for detection of Salmonella serotype Typhi by membrane filter technique (2 - 5).
Quality Control
Appearance of powder: Greenish yellow coloured, homogeneous, free flowing powder.
Colour and Clarity: Greenish yellow coloured, opaque solution which may contain flocculent precipitate.
Reaction: Reaction of 6.4% w/v aqueous solution is pH 7.7 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours, in humid atmosphere.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of colony* |
|---|---|---|---|
| Escherichia coli (25922) | 103-2x103 | inhibited | - |
| Salmonella serotype Typhi (6539) | 102-103 | luxuriant | black with sheen |
| Salmonella serotype Typhimurium (14028) | 102-103 | luxuriant | black with sheen |
| Staphylococcus aureus (25923) | 103-2x103 | inhibited | - |
Key : * = on membrane filter
| Product Name | M-Bismuth Sulphite HiVeg™ Broth |
|---|---|
| SKU | MV1101 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Clark H. F., Geldreich E. E., Jeter M. L. and Kabler P. W., 1951, Pub l. Hlth. Reports, 66:951. |
| Customized Product Available | No |
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