Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Fungi Kimmig HiVeg Agar Base is used for cultivation, identification and preservation of fungal strains.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 9.3 |
| HiVeg hydrolysate | 4.3 |
| Sodium chloride | 11.4 |
| Dextrose | 10.0 |
| Agar | 15.0 |
Final pH (at 25°C) 6.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 50 grams in 1000 ml distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, selective medium is obtained by aseptically adding filtered solutions of 0.4 gram Cycloheximide, 40,000 IU Penicillin, 40 mcg Streptomycin, 80 mg Colistin and 100 mg Novobiocin in a previously cooled sterile medium. Mix well and pour plates.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and HiVeg hydrolysate in place of Peptic digest of animal tissue and Casein enzymic hydrolysate respectively which makes the medium free of BSE/TSE risks. Fungi Kimmig HiVeg Agar Base is the modification of Fungi Kimmig Agar Base which is formulated as described by Kimmig and Rieth for the cultivation, identification and preservation of fungal strains (1). The appearance of growth on Fungi Kimmig HiVeg Agar Base, like the conventional medium, is considered as an important criteria in identification of fungal stains (2).
The medium contains HiVeg peptone and HiVeg hydrolysate which provides nitrogenous nutrients. Dextrose is the carbohydrate source while sodium chloride maintains osmotic balance of the medium. This medium can also be used as a base for preparing selective agars. Addition of antibiotics like Cycloheximide, Penicillin, Streptomycin, Colistin, Novobiocin etc. inhibit the growth of many gram-positive, gram-negative bacteria and also some fungi like Saccharomyces.
MV1010 Fungi Kimmig HiVeg Agar Base
1. Control
2. Aspergillus niger
3. Saccharomyces cerevisiae
Technical Data
Product Profile :
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1010 | M1010 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
- Recommended for: Cultivation, identification and preservation of fungal strains.
- Reconstitution: 50.0 g/l
- Quantity on preparation (500g): 10.0 L
- pH (25°C): 6.5 ± 0.2
- Supplement: Glycerol, Antibiotics, if desired
- Sterilization: 121°C / 15 minutes.
- Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
Yellow to light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Medium amber coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 5.0% w/v aqueous solution containing 0.5% v/v glylcerol is pH 6.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for upto 5 days.
| Organisms (ATCC) | Growth (without antibiotics) |
|---|---|
| Aspergillus niger (16404) | good-luxuriant |
| Candida albicans (10231) | good-luxuriant |
| Saccharomyces cerevisiae (9763) | good-luxuriant |
References
- Kimmig J. and Rieth H., 1953, Antimykotica in Experiment and Klinik, Arzneimittelforsch 3:267.
- Rieth H., 1969, Mykosen, 12:73.
| Product Name | Fungi Kimmig HiVeg™ Agar Base |
|---|---|
| SKU | MV1010 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Rieth H., 1969, Dermatophyten, Hefen und Schimmelpilze auf Kimmig-Agar. Mykosen, 12:73-74. |
| Customized Product Available | No |
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