Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Dextrose HiVeg Agar / Broth is used for cultivation of wide variety of microorganisms.
Composition**
| Ingredients | MV084 Grams/Litre |
MV044 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate No. 1 | 10.00 | 10.00 |
| HiVeg extract | 3.00 | 3.00 |
| Dextrose | 10.00 | 5.00 |
| Sodium chloride | 5.00 | 5.00 |
| Agar | 15.00 | — |
Final pH (at 25°C): MV084: 7.3 ± 0.2, MV044: 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV084/MV044 | M084/M044 |
| HiVeg hydrolysate No. 1 | Tryptose |
| HiVeg extract | Beef extract |
- Recommended for
- Cultivation of wide variety of microorganisms
- Reconstitution
-
- (MV084): 43.0 g/l
- (MV044): 23.0 g/l
- Quantity on preparation
-
- (500g): (MV084): 11.62 L
- (100g): (MV084): 2.32 L
- (500g): (MV044): 21.73 L
- (100g): (MV044): 4.34 L
- pH (25°C)
-
- (MV084): 7.3 ± 0.2
- (MV044): 7.2 ± 0.2
- Supplement
- Sterile Defibrinated Sheep Blood
- Sterilization
- 121°C / 15 minutes.
- Storage
- Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 43 grams of MV084 or 23 grams of MV044 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, Blood Agar can be prepared by the addition of 5% v/v sterile, defibrinated sheep blood into sterile Dextrose HiVeg Agar, cooled to 50°C. Mix well and despense as desired.
Principle and Interpretation
These media are prepared by completely replacing animal based peptones with vegetable peptones. Dextrose HiVeg media are the modification of Dextrose Media which are used for the cultivation of wide variety of microorganisms and specially used for making Dextrose Blood Agar (1). Dextrose HiVeg Broth like the conventional medium is used for antibiotic sensitivity testing using tube dilution method (2). This broth was found to be superior compared to Soyabean HiVeg Medium, particularly for sensitivity testing of Neomycin and Chlortetracycline.
Dextrose HiVeg Agar contains high concentration of dextrose as an energy source for the rapid growth of microorganisms. However this medium is not very suitable for the study of haemolysis because of high sugar content. HiVeg extract and HiVeg hydrolysate No. 1 serve as sources of nitrogenous compounds, sulphur, carbon, vitamins and minerals. Osmotic balance is maintained by sodium chloride.
Quality Control
Appearance of Powder
Light yellow coloured may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV084.
Colour and Clarity
Light yellow coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction
Reaction of 4.3% w/v aqueous solution of MV084 is pH 7.3 ± 0.2 at 25°C.
Reaction of 2.3% w/v aqueous solution of MV044 is pH 7.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) |
Growth (Plain) |
Growth w/blood |
Recovery w/blood |
|---|---|---|---|---|
| Bordetella pertussis (8467) | 102-103 | good-luxuriant | luxuriant | >70% |
| Neisseria meningitidis (13090) | 102-103 | good-luxuriant | luxuriant | >70% |
| Neisseria gonorrhoeae (19424) | 102-103 | good-luxuriant | luxuriant | >70% |
| Streptococcus pyogenes (19615) | 102-103 | good-luxuriant | luxuriant | >70% |
| #Clostridium perfringens (12919) | 102-103 | fair-good | luxuriant | >70% |
Key: # = Incubated anaerobically
References
- Norton, 1932, J. Lab. Clin. Med., 17:585.
- Walsbren Carr and Dunnett, 1951, Am. J. Clin. Path. 21:884.
| Product Name | Dextrose HiVeg™ Broth |
|---|---|
| SKU | MV044 |
| Customized Product Available | No |
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