Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic HiVeg Assay Medium No. 3 (Assay HiVeg Broth) is used for microbiological assay of antibiotics.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg peptone | 5.000 |
| HiVeg extract | 1.500 |
| Yeast extract | 1.500 |
| Dextrose | 1.000 |
| Sodium chloride | 3.500 |
| Dipotassium phosphate | 3.680 |
| Monopotassium phosphate | 1.320 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 17.5 grams in 1000 ml purified/distilled water. Heat with frequent agitation to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the Microbiological assay of Amikacin, Bacitracin, Capreomycin, Chlortetracycline, Chloramphenicol, Cycloserine, Demeclocycline, Dihydrostreptomycin, Doxycycline, Gentamicin, Gramicidin, Kanamycin, Lincomycin, Meclocycline, Methacycline, Minocycline, Neomycin, Novobiocin, Oxytetracycline, Rolitetracycline, Spectinomycin, Streptomycin, Tetracycline, Tobramycin, Trolendomycin, Tyrothricin, Tylosin and Viomycin according to official methods
Principle And Interpretation
Antibiotic HiVeg Assay Medium No.3 is prepared using vegetable peptones in place of animal peptones, making the medium BSE, TSE risks free. Antibiotic HiVeg Assay Medium No.3, MV042 can be used for the same purpose of Antibiotics Assay Medium No.3, used for the assay of various antibiotics. Grove and Randall have elaborately elucidated the methods to perform these assays and various media used for that (1). Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay (2). These media are also recommended by USP and FDA generally in the turbidimetric antibiotic assay of Amikacin, Bacitracin, Kanamycin, Streptomycin and other antibiotics.
Antibiotic HiVeg Assay Medium No. 3 (Assay HiVeg Broth) can also be used in the microbiological assay of different antibiotics in pharmaceutical and food products by the turbidimetric method. Ripperre et al reported that turbidimetric methods for determining the potency of antibiotics are inherently more accurate and more precise than agar diffusion procedures (2).
Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganims in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.
HiVeg Peptone, HiVeg extract and yeast extract provides essential nutrients and growth factors for enhanced microbial growth. Sodium chloride maintains the osmotic equilibrium of the medium and retains the cell viability and cell integrity. Phosphates in the medium provide good buffering action. Dextrose serves as the carbon and energy source. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important steps for the good results.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution in tubes
Reaction: Reaction of 1.75% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response: MV042: Cultural characteristics observed after an incubation at 32-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Serial dilution with |
|---|---|---|---|
| Escherichia coli ATCC 10536 | 50-100 | luxuriant | Chloramphenicol |
| Klebsiella pneumoniae ATCC 10031 | 50-100 | luxuriant | Capreomycin, Dihydrostreptomycin, Streptomycin, Troleandomycin, Neomycin |
| Enterococcus hirae ATCC 10541 | 50-100 | luxuriant | Gentamicin, Gramicidin, Neomycin, Novobiocin |
| Staphylococcus aureus ATCC 9144 | 50-100 | luxuriant | Tylosin |
| Staphylococcus aureus ATCC 29737 | 50-100 | luxuriant | Amikacin, Chlortetracycline, Cycloserine, Demeclocycline, Doxycycline, Kanamycin, Kanamycin sulphate, Methacycline, Oxytetracycline, Rolitetracycline, Tetracycline, Tobramycin, Tylosin |
Storage and Shelf Life
Store below 30°C in a tightly closed container and use freshly prepared medium. Use before expiry date on label.
| Product Name | Antibiotic HiVeg™ Assay Medium No. 3 (Assay HiVeg™ Broth) |
|---|---|
| SKU | MV042 |
| Customized Product Available | No |
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