Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| HiVeg peptone | 6.000 |
| Yeast extract | 3.000 |
| HiVeg extract | 1.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.9±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the microbiological assay of Mitomycin, Plicamycin, Vancomycin, Oxytetracycline, Tetracycline .
Principle And Interpretation
Antibiotic HiVeg Assay Medium No. 8 is prepared incorporating vegetable peptones in place of animal peptones, making the medium BSE, TSE risks free. This medium can be used for the same purpose of Antibiotic Assay Medium No. 8, for the assay of various antibiotics. Grove and Randall have elaborately elucidated the methods to perform these assays and various media used for the same (1). Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay (2). These media are also recommended by USP (3) and FDA (4). Similarly Antibiotic HiVeg Assay Medium No. 8, MV041 is used especially to prepare the base layer to assay Tetracyclines and other antibiotics. It provides a solidified substratum for growth of organisms. The medium has an optimal pH of 5.9 for assay of Tetracycline as these antibiotics are stable at slightly lower pH (5). This pH condition also supports the growth of test organisms. Antibiotic HiVeg Assay Medium No. 8 is also used as base and seed agar medium for agar diffusion assay for Mitomycin, Mithramycin, Plicamycin and Vancomycin.HiVeg extract, Yeast extract and HiVeg Peptone serves as a source of nutrients and growth factors. Note: For Antibiotic Assay Methods and Selection of Antibiotic HiVeg Assay Medias, Refer Section Antibiotic HiVeg Assay Media.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured slightly opalescent gel forms in Petriplates
Reaction
Reaction of 2.55% w/v aqueous solution at 25°C. pH : 5.9±0.2
pH
5.70-6.10
Cultural Response
MV041: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | >=70% | Mitomycin, Vancomycin |
| Bacillus cereus var mycoides ATCC 11778 | 50-100 | luxuriant | >=70% | Oxytetracycline, Tetracycline |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on label.
| Product Name | Antibiotic HiVeg™ Assay Medium No. 8 (Base HiVeg™ Agar w/ low pH) |
|---|---|
| SKU | MV041 |
| Customized Product Available | No |
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