Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium No. 41
For the microbiological assay of Thiostrepton using Enterococcus hirae as the test organism in accordance with US Pharmacopoeia.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone # | 9.000 |
| Yeast extract | 5.000 |
| Dextrose | 20.000 |
| Dibasic potassium phosphate | 1.000 |
| Monobasic potassium phosphate | 1.000 |
| Sodium citrate | 10.000 |
| pH after sterilization | 6.8±0.1 |
**Formula adjusted, standardized to suit performance parameters
#Pancreatic digest of casein
Directions
Suspend 46.0 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is formulated in accordance with USP(1). This medium is used for turbidimetric microbiological assay of Thiostreptone, a polypeptide antibiotic.
Essential amino acids, mineral and growth factors are supplied by tryptone and yeast extract in this medium. Dextrose provides carbon and energy source for enhancing the growth of test organism. Good buffering action is maintained by phosphates in the medium. Sodium citrate provides additional source of carbon and energy and promote enhanced growth of the test organism.
Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganism in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate
Reaction: Reaction of 4.6% w/v aqueous solution. pH: 6.8±0.1
pH: 6.70-6.90
Cultural Response
MU1144: Cultural characteristics observed after an incubation at 36-37.5°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Serial dilution with |
|---|---|---|---|
| Enterococcus hirae ATCC 10541 | 50-100 | luxuriant | Thiostrepton |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label
| Product Name | Antibiotic Assay Medium No. 41 |
|---|---|
| SKU | MU1144 |
| Customized Product Available | No |
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