Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium No. 40
For the microbiological assay of Thiostrepton using Enterococcus hirae as the test organism in accordance with US Pharmacopoeia.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Polypetone | 5.000 |
| Yeast extract | 20.000 |
| Dextrose | 10.000 |
| Monobasic potassium phosphate | 2.000 |
| Polysorbate 80 | 0.100 |
| Agar | 10.000 |
| pH after sterilization | 6.7±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.1 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
This medium is formulated in accordance to USP (1).This medium is used as maintenance medium for test organism Enterococcus hirae ATCC 10541 used for assay of Thiostrepton. Essential amino acids, mineral and growth factors are supplied by polypeptone and yeast extract in this medium. Dextrose functions as carbon and energy source for enhancing the growth of test organism. During maintenance of the test organims, good buffering action is provided by phosphates in the medium. Incorporation of polysorbates reduces the surface tension, maintaining uniform suspension of cells and can also neutralize phenolic compounds in the test sample, if any.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.0% Agar gel
Colour and Clarity of prepared medium: Light amber coloured slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 4.71% w/v aqueous solution. pH: 6.7±0.2
pH: 6.50-6.90
Growth Promotion Test
As per United States Pharmacopoeia
Cultural Response: MU1143: Cultural characteristics observed after an incubation at 36-37.5°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Enterococcus hirae ATCC 10541 | 50-100 | luxuriant | >=70% | Thiostrepton |
Storage and Shelf Life
Store below 30°C in tightly container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium No. 40 |
|---|---|
| SKU | MU1143 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. United States Pharmacopoeia 2009,US Pharmacopoeial Convention, Inc., Rockville, MD. |
| Customized Product Available | No |
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