Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium No. 39
For the microbiological assay of Neomycin using Klebsiella pneumoniae and Tylosin using Staphylococcus aureus as the test organism in accordance with US Pharmacopoeia.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 5.000 |
| Yeast extract | 1.500 |
| Beef extract | 1.500 |
| Dextrose | 1.000 |
| Sodium chloride | 3.500 |
| Dibasic potassium phosphate | 3.680 |
| Monobasic potassium phosphate | 1.320 |
| pH after sterilization | 7.9±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 17.5 grams in 1000 ml purified/distilled water. Heat if necessary todissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Antibiotic Assay media are used in the performance of antibiotic assays. Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays (1). Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay (2). This medium is prepared in accordance with the USP (3) and the FDA (4).
Nutrients and growth factors are provided by ingredients like peptone, beef extract and yeast extract. Dextrose is the source of energy. Sodium chloride maintains the osmotic equilibrium whereas the phosphates act as the buffering system
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate
Reaction: Reaction of 1.75% w/v aqueous solution (after sterilization). pH: 7.9±0.1
pH: 7.80-8.00
Cultural Response: MU1142: Cultural characteristics observed after an incubation at specified temperature for specified time
| Organism | Inoculum (CFU) | Growth | Serial dilution with | Incubation temp/ period |
|---|---|---|---|---|
| Klebsiella pneumoniae ATCC 10031 | 50-100 | luxuriant | Neomycin | 36-37.5°C/ 16-24 hours |
| Staphylococcus aureus ATCC 9144 | 50-100 | luxuriant | Tylosin | 36-37.5°C/ 16-18 hours |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label
| Product Name | Antibiotic Assay Medium No. 39 |
|---|---|
| SKU | MU1142 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Grove and Randall, 1955, Assay Methods of Antibiotics Medical Encyclopedia, Inc,New York. |
| Customized Product Available | No |
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