Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium No. 32
For preparing inoculum of Bacillus subtilis to be used as test organism for assaying Dihydrostreptomycin and Vancomycin by plate assay method in accordance with US Pharmacopoeia.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 6.000 |
| Pancreatic digest of casein | 4.000 |
| Yeast extract | 3.000 |
| Beef extract | 1.500 |
| Dextrose | 1.000 |
| Manganese sulphate | 0.300 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.6±0.1 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.8 gms in 1000ml purified/distilled water. Heat to boiling to disslove the medium completely. Dispense and sterilise by autoclaving at 15lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the microbiological assay of Dihydrostreptomycin and Vancomycin
Principle And Interpretation
This medium is formulated in accordance to USP and FDA (1,2) and is a modification of Antibiotic assay medium No.1. This medium is used to develop incoulum of Bacillus subtilis for antibiotic assay.
Essential nutrients, vitamins, mineral, trace elements and growth factors are supplied by peptone, pancreatic digest of casein, yeast and beef extract. Dextrose in the medium serves as the carbon source for stimulating the growth of the test microorganism.
Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis (3,4,5), which is generally used as test organisms for plate assay of Dihydrostreptomycin and Vancomycin .
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow coloured clear to slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 3.08% w/v aqueous solution. pH: 6.6±0.1
pH: 6.50-6.70
Cultural Response
MU1141: Cultural characteristics observed after an incubation at 32-35°C for 5 days.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | good-luxuriant | >=70% | Dihydrostreptomycin, Vancomycin |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium No. 32 |
|---|---|
| SKU | MU1141 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983 Title 21, Part 436, SubpartD, Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242-259, (April1). ,, |
| Customized Product Available | No |
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