Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Antibiotic Assay Medium No. 19
For microbiological assay of Amphotericin B, Natamycin and Nystatin using Saccharomyces cerevisiae as the test organisms in accordance with US Pharmacopoeia.
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 9.400 |
| Yeast extract | 4.700 |
| Beef extract | 2.400 |
| Dextrose | 10.000 |
| Sodium chloride | 10.000 |
| Agar | 23.500 |
| pH after sterilization | 6.1±0.1 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 60.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile Petri plates.
Principle And Interpretation
The medium composition is in accordance to USP and CFR (1,2). This medium is used as seed agar for assay of antifungal agents like Amphotericin B and Nystatin. This medium is used for maintenance and inoculum development of Saccharomyces cerevisiae. This medium is also used for assaying mycostatic activity in pharmaceutical formulations. This medium is forumulated as reported by Kirshbam and Arret (3).
Ingredients like peptone, yeast and beef extract supplement essential nutrients, minerals and growth factors for the growth of test organism. Dextrose in the medium provides enhanced source of carbon and energy. Osmotic equilibrium in the medium is maintained by sodium chloride which retains the cell intergrity and viability.Antibiotic assay medium No.19, is used as both base and seed medium for agar diffusion assay for antibiotics like Amphotericin B and Nystatin.
Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar precooled to 40-45°C and spread evenly over the surface of solidified base agar. Prediffusion of antibiotics for 20 minutes in the agar by incubating at temperature below the optimal growth temperature for microorganism would facilitate better diffusion of antibiotic, followed by incubation of the plates for microbial growth.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 2.35% Agar gel.
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
pH
6.00-6.20
Growth Promotion Test
As per United States Pharmacopoeia.
Cultural Response
Cultural characteristics observed after an incubation at 29-31°C for 24-48 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Saccharomyces cerevisiae ATCC 2601 | 50-100 | luxuriant | >=70% | Nystatin |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | >=70% | Amphotericin B |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium . Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium No. 19 |
|---|---|
| SKU | MU101 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Grove and Randall, 1955, Assay Methods of Antibiotics Medical Encyclopedia, Inc,New York. 2.Schmidt and Moyer, 1944; J. Bact, 47:199. 3.United States Pharmacopoeia 200 4.Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 198 3.Title 21, part 436, SubpartD, Washington, D.C. U.S Government printing office, paragraphs 436, 100-436, 106 pg 242-259 (April 1). 5.Krishbaum A and Areet B, 1967, J. Pharm Sci, 56: 512. |
| Customized Product Available | No |
Write Your Own Review
Antibiotic HiVeg™ Assay Medium No. 19
As low as
₹6,832.00
Antibiotic Assay Medium No. 19
As low as
₹6,508.00
Antibiotic Assay Medium G
As low as
₹0.00