Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for detection of contamination in areas of environmental monitoring.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tube 1-0.9% Saline | |
| Sodium chloride | 9.000 |
| Distilled water | 1000.000 ml |
| Tube 1 - SCDM w/BCP | |
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Bromocresol purple | 0.010 |
| Distilled water | 1000.000 ml |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab provided along with the Saline containing tube, collect the sample or specimen from surface. Discard the cap of the second tube and insert the capped swab with the sample in second tube containing SCDM w/BCP. Tighten the cap firmly. Observe the results after
Principle And Interpretation
Soyabean Casein Digest Medium is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi (1-4). It can also be used as a general, all purpose cultivation medium (1). When tested for the growth of organisms in presence of indicator like bromocresol purple, the colour of the medium changes from purple to yellow. With the addition of carbohydrates it can be also used for the fermentation studies of fastidious and non-fastidious organisms. Tryptone and soya peptone provides necessary amino acids and other complex nitrogenous substances. Dextrose serves as an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH.
Type of specimen
Environmental monitoring- Sterility testing in manufacturing process, Pharmaceutical samples
Specimen Collection and Handling:
For pharmaceutical samples, follow appropriate techniques for sample collection and processing as per guidelines (1-4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Users are recommended to validate the medium for any specific microorganisms other than mentioned in the certificate of analysis based on users unique requirement, as each lot has been tested for the organisms specified on the certificate of analysis.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Each pack contains two tubes, HiCulture™ Sterile Swabs w/0.9% Saline in one tube & second tube containing HiCulture™ Sterile Swabs w/ SCDM w/BCP.
Colour: 0.9% Saline: Colourless clear solution, SCDM w/BCP : Light purple to purple clear solution
Quantity of Medium: 5 ml of medium in tubes
Reaction: 7.10-7.50
Sterility test: Passes release criteria
Cultural response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Recovery |
|---|---|---|
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant |
Key: (#) Formerly known as Aspergillus niger, (*) Corresponding WDCM numbers
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 5-25°C. Use before expiry date on the label. Product performance is best if used within stated expiry period
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Hiculture™ Sterile Swabs w/ 0.9% saline & second tube containing SCDM w/ BCP |
|---|---|
| SKU | MS5430 |
| Customized Product Available | No |
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