Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for determination of bacterial activity of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus ATCC 6538.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 10.000 |
| HM peptone B # | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 | 5.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Using the capped swab provided along with the Saline containing tube, collect the sample or specimen from surface. Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature for more than 24 hours.
Principle And Interpretation
Letheen Broth was developed by Quisno, Gibby and Foter (2) by the addition of lecithin and Polysorbate 80 to FDA Broth. Letheen Broth is recommended by AOAC to determine the phenol coefficient of cationic surfactants (3). Letheen Medium is also recommended for testing of cosmetics (4).
HM Peptone B and peptone supply nitrogenous and carbonaceous compounds, long chain amino acid, essential nutrients and other trace elements for the microbial growth. Lecithin and polysorbate 80 enables the recovery of bacteria from solutions containing residues of disinfectant used in sanitization of utensils and equipments. Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene and formalin (5, 1). Dehydrated medium may appear moist with brown sugar appearance, which does not indicate deterioration (1).
Type of specimen
Enviromental samples; Water samples
Specimen Collection and Handling
For enviromental samples, follow appropriate techniques for sample collection and processing as per guidelines (7).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1)
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to nutritional variation certain strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile HiCulture™ Sterile Swabs w/Letheen Broth
Colour
Yellow clear solution
Quantity of Medium
5 ml of medium in tubes
Reaction
6.80-7.20
Sterility test
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | good-luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 5-25°C. Use before expiry date on the label. Product performance is best if used within stated expiry period
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | HiCulture™ Sterile Swabs w/ Letheen Broth |
|---|---|
| SKU | MS5397 |
| Customized Product Available | No |
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