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HiCulture™ Sterile Swabs w/ 0.9% Saline
Intended use
Recommended for use as a diluent and collection of specimens from surface.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Sodium chloride | 9.000 |
| Distilled water | 1000.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab provided along with the Saline containing tube, collect the sample or specimen from surface. Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will dimish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The culture on transport swabs must not be kept at room temperature for more than 24 hours.
Quality control
Appearance: Sterile 0.9% saline in tubes w/ sterile cotton swabs.
Colour: Clear colourless solution
Quantity: 10 ml of solution in tubes.
Reaction: Reaction pH of medium is 7.00
Sterility check: Passes release criteria
Cultural Response
Viability of various microorganisms was established for a period upto 24 hours. Organisms grew luxuriantly when inoculated and recovered on Tryptone Soya Agar (M290) and incubated at 35 -37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf life
Store between 5-25°C with caps firmly screwed.. Use before expiry date on the label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiCulture™ Sterile Swabs w/ 0.9% Saline |
|---|---|
| SKU | MS5321 |
| Customized Product Available | No |

