Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for recovery of Candida albicans from clinical specimens.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Ammonium sulphate | 1.000 |
| Potassium dihydrogen phosphate | 1.000 |
| Biotin | 0.000005 |
| Trypan blue | 0.100 |
| Purified polysaccharide | 20.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab provided along with the medium containing tube, collect the sample or specimen from surface. Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature for more than 24 hours.
Principle And Interpretation
Effective recovery of microorganisms and its identification is dependent on a number of factors such as collection and transportation to the laboratory under conditions which allow maintenance of viability. Chlamydospore medium is prepared according to the formula of Nickerson and Mankowski (3). The medium is chemically defined, semisolid supporting the survival of Candida species for a sufficiently long period. Sterile cotton swabs allow absorption of specimen material while polystyrene shaft allows semiflexibility to the swab stick, aiding in collection.
Ammonium sulphate acts as sources of ions that simulate metabolism. Potassium dihydrogen phosphate provides buffering to the medium. Biotin provides the necessary vitamins required for metabolism. Purified polysaccharide acts as a source of carbon. Trypan blue is a vital dye absorbed selectively by the chlamydospores and imparts blue colour to chlamydospores, whereas the filaments are colourless.
Type of specimen
Clinical samples; Environmental monitoring
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Users are recommended to validate the medium for any specific microorganisms other than mentioned in the certificate of analysis based on users unique requirement, as each lot has been tested for the organisms specified on the certificate of analysis.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Chlamydospore Medium in tubes with sterile viscous swabs with metal stick
Colour
Blue coloured medium
Quantity of Medium
8ml of medium in tubes
Reaction
4.90-5.30
Sterility check
Passes release criteria
Cultural response
Viability of following organism was established for a period of 48 hours.Organisms grew luxuriantly and Chlamydospore formation was observed when recoverd on Sabouraud Dextrose Agar(M063) and incubated at 22 - 28°C for 48 hours.
| Organism | Growth | Chlamydospores |
|---|---|---|
| Candida albicans ATCC 10231 (00054*) | good | positive |
| Candida kruisei ATCC 24408 | good | negative |
| Candida minosa | good | negative |
| Candida tropicalis ATCC 1369 | good | negative |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 5-25°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiCulture™ Transport Swabs w/ Chlamydospore Medium with metal stick |
|---|---|
| SKU | MS113S |
| Customized Product Available | No |
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