Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended as a diluent and for general use for collection of specimens from surface.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Sodium chloride | 8.500 |
| Distilled water | 1000.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab provided along with the Saline containing tube, collect the sample or specimen from surface. Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature for more than 24 hours.
Type of specimen
Environmental samples
Specimen Collection and Handling
For Environmental samples follow appropriate techniques for handling specimens as per established guidelines (1,2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile 0.85% Saline in tube with Sterile Cotton Swabs (gamma-irradiated) (Triple pack).
Colour
Clear colourless solution
Quantity of Medium
8ml of medium in tubes
Reaction
7.00-7.00
Sterility test
Passes release criteria
Dose of Irradiation (Kgy)
15.00-25.00
Cultural Response
Viability of following was established for a period of 24 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35-37°C for 18-24 hours.
| Organism | Recovery |
|---|---|
| Escherichia coli ATCC 25922 (00013*) | Luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | Luxuriant |
| Psuedomonas aeruginosa ATCC 27853 (00027*) | Luxuriant |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 5-25°C. Use before expiry date on the label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiCulture™ Sterile Swabs w/ 0.85% Saline (γ-irradiated) (Triple Pack) |
|---|---|
| SKU | MS001GT |
| Customized Product Available | No |
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