Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For the subculture of fungi in accordance with the harmonized method of USP/EP/BP/JP.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Infusion from potatoes | 200.000 |
| Dextrose | 20.000 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (4). It is also recommended by USP (5), BP (6) ,EP (7) and JP (8) for growth of fungi. Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify.
Quality Control
Appearance: Sterile Potato Dextrose Agar in 90 mm disposable plate.
Colour: Light amber coloured medium.
Quantity of Medium: 25ml of medium in plates.
pH: 5.40-5.80
Growth Promotion Test
Growth Promotion was carried out in accordance with the harmonized method of USP/EP/BP/JP, and growth was observed at 20 -25°C for specified time. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose Agar
Cultural Response
MPH096: Cultural characteristics was observed after an incubation at 20 -25°C for 2-5 days. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose Agar (*- Formerly known as Aspergillus niger)
| Organism | Inoculum (CFU) | Growth | Lot value (CFU) | Recovery | Ascospore formation | Sterility Testing |
|---|---|---|---|---|---|---|
| Growth after 2-3 days | ||||||
| Candida albicans ATCC 10231 | 50-100 | luxuriant | 35-100 | >=70% | ||
| Growth after 5-7 days | ||||||
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant(for 5-7 days) | ||||
| Additional Microbiological Testing | ||||||
| Trichophyton rubrum ATCC 28191 | ||||||
| Rhodotorula mucilaginosa DSM 70403 | 50-100 | luxuriant | ||||
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | 35-100 | >=70% | positive | |
| Geotrichum candidum DSM 1240 | good- luxuriant | |||||
| Penicillium communae ATCC 10248 | fair -good | |||||
| Trichophyton ajelloi ATCC 28454 | fair-good | |||||
| Sterility Test | Passes release criteria. | |||||
Storage and Shelf Life
Store at 15-25°C. Use before expiry date on the label. *Key:-Formerly known as ! Aspergillus niger @ ATCC 16404
| Product Name | Potato Dextrose Agar Plate |
|---|---|
| SKU | MPH096 |
| Customized Product Available | No |
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