Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for selective isolation of Campylobacter species from faecal specimens, food and environmental specimens.
Composition**
| Ingredients | g/L |
|---|---|
| Proteose peptone | 15.000 |
| HML extract # | 2.500 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 12.000 |
| Sheep Blood | 100ml |
| Final pH (at 25°C) | 7.4±0.2 |
Blaser-Wang Selective Supplement (FD006)
2 vials
| *Ingredients | Concentration |
|---|---|
| Polymyxin B | 1.250IU |
| Vancomycin | 5mg |
| Trimethoprim | 2.500mg |
| Amphotericin B | 1mg |
| Cephalothin | 7.500mg |
**Formula adjusted, standardized to suit performance parameters
#Equivalent to Liver digest
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Campylobacter species are ubiquitous in the environment inhabiting a wide variety of ecological niches (1). Infection with a Campylobacter species is one of the most common causes of human bacterial gastroenteritis (1). Most species are found in animals (cattle, swine) and cause infertility and abortion (2). C.jejuni was originally isolated on a blood-containing media with antibiotics (3). Skirrow described a selective medium for Campylobacter species consisting of Blood Agar Base No. 2 supplemented with horse blood and antibiotics (4). Subsequently, Blaser et al isolated C.jejuni on Brucella Agar supplemented with sheep blood and four antibiotics (5). Later on, a fifth antibiotic, cephalothin was added to improve the selectivity of the medium by inhibition of accompanying faecal bacteria (6). Campylobacter Agar Base is recommended by APHA for selective isolation of Campylobacter species (7).
Campylobacter Agar Base is well supplemented to support luxuriant growth of Campylobacter species. Osmotic equilibrium of the medium is maintained by sodium chloride. Blood serves as an additional source of nutrients including X-factor. The antibiotic supplements namely Blaser-Wang (FD006) and Skirrow (FD008) markedly reduce the growth of normal enteric bacteria while enhancing the growth and recovery of C.jejuni from faecal specimens. Amphotericin B in Blaser-Wang supplement greatly or completely inhibits growth of fungi. C.jejuni colonies appear non-haemolytic, flat and gray with an irregular edge or raised and round with a mucoid appearance. Some strains may appear tan or slightly pink. Swarming may be observed on moist surfaces. Incubation at 35-37°C may show a delayed growth of C.jejuni cultures. Incubating the plates at 42°C can fasten this.
The contaminated food sample (10 to 25 grams) is enriched in Campylobacter Enrichment Broth Base (M899 + FD042). The broth is incubated with agitation under a micro aerobic atmosphere for 16-18 hrs. The enrichment culture is then plated onto the selective media i.e. Campylobacter Agar Base (M994) (7).
Type of specimen
Clinical samples - Faeces; Food and dairy samples; Environmental samples.
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (8,9).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (10,11). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitation
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further serological and biochemical testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Campylobacter Agar in 90 mm disposable plates with smooth surface & absence of black particles/ cracks/ bubbles.
Colour
Red coloured medium
Quantity of medium
25ml of medium in 90mm disposable plate
Sterility Check
Passes release criteria
pH
7.20-7.60
Cultural Response
Cultural characteristics observed under reduced oxygen atmosphere after an incubation at 35-37°C for 24-48 hours.
| Organism | Growth |
|---|---|
| Candida albicans ATCC 10231 (00054*) | none - poor |
| Campylobacter jejuni ATCC 29428 (00156*) | good-luxuriant |
| Escherichia coli ATCC 25922 (00013*) | none - poor |
| Enterococcus faecalis ATCC 29212 (00087*) | none - poor |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (10,11).
| Product Name | Campylobacter Agar Plate |
|---|---|
| SKU | MP994 |
| Customized Product Available | No |
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