Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended use
Recommended for selective enumeration and identification coliform organisms.
Composition**
| Ingredients | g/L |
|---|---|
| Proteose peptone | 5.000 |
| Yeast extract | 3.000 |
| Lactose | 10.000 |
| Sodium heptadecyl sulphate (Tergitol 7) | 0.100 |
| Bromo thymol blue | 0.025 |
| Agar | 15.000 |
| TTC Solution 1% (10 ml per vial) (FD057) | 3 ml |
| Final pH (at 25°C) | 6.9±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Tergitol-7 Agar was originally designed by Chapman (1) and later on modified by incorporating 2,3,5-Triphenyl Tetrazolium Chloride (TTC) into the medium. This medium is selective and differential used for the detection and enumeration of coliform organisms. Pollard (2) has reported the selective bactericidal property of sodium heptadecyl sulphate (Tergitol-7). Kulp et al (3) corroborated the use of Tergitol-7 Agar with TTC in routine analysis of water and Mossel (4) used this medium for the examination of food materials.
Proteose peptone and yeast extract serve as sources of carbon, nitrogen and other essential nutrients including vitamin B complex. Sodium heptadecyl sulphate (Tergitol-7) inhibits gram-positive bacteria and Proteus swarming and yields better recovery of coliforms. Bromo thymol blue is the pH indicator. Lactose fermenting organisms form yellow colonies with yellow zones while Klebsiella and Enterobacter form greenish yellow colonies. Lactose non-fermenters produce blue colonies. TTC is reduced by the bacterial cell except Escherichia coli and Enterobacter aerogenes to form formazan, a red coloured insoluble complex, thereby producing red coloured colonies.
Filter the specimen to be analyzed through two membranes. Place the membrane upon two TTC Tergitol Agar plates. Incubate one plate at 37°C for 24 hours (total coliforms) and the other at 44°C for 18-24 hours (faecal coliforms). The yellow colonies with deep yellow halo after incubation at 44°C should be identified as faecal coliform bacteria.
Type of specimen
Clinical samples - faeces, urine and other pathological material; Water samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Tergitol-7 Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Green coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
6.70-7.10
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony/ medium |
|---|---|---|---|---|
| #Klebsiella aerogenes ATCC 13048 (00175*) |
50-100 | luxuriant | >=50% | Reddish brown |
| Escherichia coli ATCC 25922 (00013*) |
50-100 | good-luxuriant | >=50% | yellow with red centre |
| Proteus mirabilis ATCC 25933 |
50-100 | good | 40-50% | red with bluish zone |
| Pseudomonas aeruginosa ATCC 27853 (00025*) |
50-100 | good | 40-50% | red with bluish zone |
| Salmonella Typhimurium ATCC 14028 (00031*) |
50-100 | luxuriant | >=50% | red with bluish zone |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) |
>=103 | inhibited | 0% | |
| Shigella flexneri ATCC 12022 (00126*) |
50-100 | good-luxuriant | >=50% | red with bluish zone |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Tergitol-7 Agar Plate |
|---|---|
| SKU | MP616 |
| Customized Product Available | No |
Write Your Own Review