Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the selective isolation of yeasts, moulds and aciduric bacteria from clinical and non-clinical specimens.
Composition
| Ingredients | g/L |
|---|---|
| Dextrose (Glucose) | 40.000 |
| Mycological, peptone | 10.000 |
| Agar | 15.000 |
| Cycloheximide | 500mg |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Sabouraud Dextrose Agar is Carliers modification (1) of the formulation described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (3). Mycological peptone provides nitrogenous compounds. Dextrose (Glucose) provides an energy source. High dextrose concentration and low pH favours fungal growth and inhibits contaminating bacteria from test samples (4). Cycloheximide inhibits some saprophytic and pathogenic fungi. Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet. For heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.
Type of specimen
Clinical samples - Skin scrappings, nail scrappings
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets
Limitations
- Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical tests should be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Sabouraud Dextrose Agar w/ Cycloheximide in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of Medium: Light amber coloured medium.
Quantity of Medium: 25ml of medium in 90 mm disposable plates
pH: 5.40-5.80
Growth Promotion Test: Growth Promotion was carried out in accordance with the standard method and growth was observed after a specified period. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose Agar.
Sterility check: Passes release criteria
Cultural Response: Cultural characteristics observed after incubation at 20-25 °C for 2-3 days.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | fair-good |
| *Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | none-poor(for 5-7 days) |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | none-poor |
| Trichophyton rubrum ATCC 28191 | luxuriant(for 5-7 days) | |
| $Trichophyton interdigitale ATCC 9533 | luxuriant(for 5-7 days) |
Key: (*): Corresponding WDCM numbers.
(*): Formerly known as Aspergillus niger ($): Formerly known as Trichophyton mentagrophytes.
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Sabouraud Dextrose Agar Plate w/ Cycloheximide |
|---|---|
| SKU | MP5387 |
| Customized Product Available | No |
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